cDNA cloning and characterization of a human sperm antigen (SPAG6) with homology to the product of the Chlamydomonas PF16 locus
cDNA cloning and characterization of a human sperm antigen (SPAG6) with homology to the product of the Chlamydomonas PF16 locus
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DOI：
10.1006/geno.1999.5914
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发表时间：
1999-09-15
期刊：
影响因子：
4.4
通讯作者：
Strauss, JF
中科院分区：
文献类型：
--
作者：
Neilson, LI;Schneider, PA;Strauss, JF
从一个高滴度抗精子抗体的不育男性血清中，通过筛选睾丸表达文库，鉴定了一种新的人精子抗原。该克隆最初命名为Repro-SA-1（HUGO批准的符号SPAG 6），发现其编码在睾丸中高度富集的序列。推导的全长cDNA的氨基酸序列显示与莱茵衣藻PF 16基因座的产物具有惊人的同源性，该基因编码位于鞭毛轴丝中心对的蛋白质。人类基因编码1.8-和2.8-kb的mRNA，在睾丸中高度表达，但在前列腺、卵巢、脾脏、胸腺、小肠、结肠、外周血白细胞、心脏、脑、胎盘、肝脏肌肉肾脏和胰腺该基因定位于染色体10p11.2-p12。针对SPAG 6序列产生的抗体将蛋白质定位于透化的人精子的尾部。衣原体蛋白和SPAG 6都含有8个连续的犰狳重复序列，这将它们置于已知介导蛋白质-蛋白质相互作用的蛋白质家族中。克隆衣原体PF 16基因座的人类同源基因为探索轴丝中心对在人类精子功能中的作用提供了新的途径。(C)北京：科学出版社.
Serum from an infertile male with high-titer antisperm antibodies was used to identify a novel human sperm antigen by screening of a testis expression library. The clone, initially designated Repro-SA-1 (HUGO-approved symbol SPAG6), was found to encode a sequence highly enriched in testis. The deduced amino acid sequence of the full-length cDNA revealed striking homology to the product of the Chlamydomonas reinhardtii PF16 locus, which encodes a protein localized to the central pair of the flagellar axoneme, The human gene encodes 1.8- and 2.8-kb mRNAs highly expressed in testis but not in prostate, ovary, spleen, thymus, small intestine, colon, peripheral blood leukocytes, heart, brain, placenta, liver, muscle, kidney, and pancreas. The gene was mapped to chromosome 10p11.2-p12. Antibodies raised against SPAG6 sequences localized the protein to the tails of permeabilized human sperm. Both the Chlamydomonas protein and SPAG6 contain eight contiguous armadillo repeats, which place them in a family of proteins known to mediate protein-protein interactions. The cloning of the human homologue of the Chlamydomonas PF16 locus provides a new avenue to explore the role of the axoneme central pair in human sperm function. (C) 1999 Academic Press.