The WASP-Arp2/3 complex signal cascade is involved in actin-dependent sperm nuclei migration during double fertilization in tobacco and maize.
The WASP-Arp2/3 complex signal cascade is involved in actin-dependent sperm nuclei migration during double fertilization in tobacco and maize.
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DOI：
10.1038/srep43161
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发表时间：
2017-02-22
影响因子：
4.6
通讯作者：
Sun M
中科院分区：
文献类型：
--
作者：
Peng X;Yan T;Sun M
最近发现拟南芥和水稻受精过程中的精子核迁移是肌动蛋白依赖的，但这种肌动蛋白细胞骨架依赖运动背后的驱动力尚不清楚。本研究证实了植物受精过程中肌动蛋白依赖的精子核迁移是一种保守的机制。利用体外受精系统，我们发现一种功能性肌动蛋白在玉米和烟草配子膜融合后的精子核迁移中也是必不可少的。细胞骨架解聚抑制剂支持双受精过程中卵细胞和中央细胞的精核迁移依赖于肌动蛋白而不依赖于微管的观点。我们进一步揭示了基于肌动蛋白的运动肌凝蛋白不是玉米和烟草精子核迁移的驱动力。WASP-Arp2/3复合信号级联参与了玉米和烟草精子核迁移的调控。有趣的是，体细胞内的精子核迁移还需要WASP-Arp2/3复合体信号级联和肌动蛋白，这表明精子核迁移的机制不是配子特异性的。
Sperm nuclear migration during fertilization in Arabidopsis and rice has recently been found to be actin-dependent, but the driving force behind this actin cytoskeleton-dependent motion is unclear. Here, we confirmed that the actin-dependent sperm nuclei migration during fertilization is a conserved mechanism in plants. Using in vitro fertilization systems, we showed that a functional actin is also essential in maize and tobacco for sperm nuclei migration after gamete membrane fusion. Cytoskeleton depolymerization inhibitor treatments supported the view that sperm nuclei migration is actin-dependent but microtubule-independent in both egg cell and central cell during double fertilization. We further revealed that the actin-based motor myosin is not the driving force for sperm nuclear migration in maize and tobacco. The WASP-Arp2/3 complex signal cascade is shown here to be involved in the regulation of sperm nuclear migration in maize and tobacco. It is interesting that sperm nuclei migration within somatic cell also need WASP-Arp2/3 complex signal cascade and actin, suggesting that the mechanism of sperm nuclear migration is not gamete specific.