Author Correction: Control of in vivo ictogenesis via endogenous synaptic pathways.
Author Correction: Control of in vivo ictogenesis via endogenous synaptic pathways.
复制标题
作者更正：通过内源性突触途径控制体内炎症发生。
DOI：
10.1038/s41598-017-17038-1
复制
发表时间：
2017
影响因子：
4.6
通讯作者：
Stacey,WilliamC
中科院分区：
文献类型：
--
作者：
Luna-Munguia,Hiram;Starski,Phillip;Chen,Wu;Gliske,Stephen;Stacey,WilliamC
癫痫发作的随机性给癫痫研究带来了困难的挑战。非常需要一种可靠的方法来控制癫痫发作的途径，这将允许研究癫痫发作的机制和优化治疗。我们的假设是，癫痫病灶内随机传入突触活动（即突触噪声）的增加是癫痫发作的一种内源性方法。基于先前的理论和体外研究表明突触噪声可以诱导癫痫发作，我们建立了一个新的癫痫发作的活体模型。通过增加海马体的传入连接的兴奋性，我们可以控制特定时间段内颞叶癫痫发作的风险。海马中的传入突触活动通过向重聚核局灶性微量注射氯化钾来调节，在此期间癫痫发作发生的风险大幅增加。诱导的癫痫发作在定性和定量上与自发性癫痫发作难以区分。因此，该模型允许通过内源性途径直接控制颞叶癫痫发作阈值，提供了一种新的工具，用于研究癫痫发作的机制和生物标志物，测试癫痫发作阈值，并快速调整抗癫痫治疗。
The random nature of seizures poses difficult challenges for epilepsy research. There is great need for a reliable method to control the pathway to seizure onset, which would allow investigation of the mechanisms of ictogenesis and optimization of treatments. Our hypothesis is that increased random afferent synaptic activity (i.e. synaptic noise) within the epileptic focus is one endogenous method of ictogenesis. Building upon previous theoretical andin vitrowork showing that synaptic noise can induce seizures, we developed a novelin vivomodel of ictogenesis. By increasing the excitability of afferent connections to the hippocampus, we control the risk of temporal lobe seizures during a specific time period. The afferent synaptic activity in the hippocampus was modulated by focal microinjections of potassium chloride into thenucleus reuniens, during which the risk of seizure occurrence increased substantially. The induced seizures were qualitatively and quantitatively indistinguishable from spontaneous ones. This model thus allows direct control of the temporal lobe seizure threshold via endogenous pathways, providing a novel tool in which to investigate the mechanisms and biomarkers of ictogenesis, test for seizure threshold, and rapidly tune antiseizure treatments.