Enhanced conversion of induced neuronal cells (iN cells) from human fibroblasts: Utility in uncovering cellular deficits in mental illness-associated chromosomal abnormalities
Enhanced conversion of induced neuronal cells (iN cells) from human fibroblasts: Utility in uncovering cellular deficits in mental illness-associated chromosomal abnormalities
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DOI：
10.1016/j.neures.2015.07.011
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发表时间：
2015-12-01
影响因子：
2.9
通讯作者：
Ishizuka, Koko
中科院分区：
文献类型：
--
作者：
Passeri, Eleonora;Wilson, Ashley M.;Ishizuka, Koko
诱导神经元细胞（iN细胞）的新技术在转化神经科学中很有前景，因为它允许将人类成纤维细胞转化为具有有丝分裂后神经元特征的细胞。然而，一个主要的技术障碍是低转化率。为了克服这个问题，我们对转换介质进行了优化。使用我们改进的配方，我们研究了主要精神疾病相关的染色体异常如何影响iN细胞的特征。我们证明，我们的新iN细胞培养方案使我们能够获得比以前的iN细胞方法更精确的神经细胞表型测量。因此，这种iN细胞培养提供了一个平台，可以有效地获得由遗传差异引起的可能的细胞表型，这可以在使用其他人类细胞模型（如诱导多能干细胞）的研究中更深入地研究。(C) 2015爱思唯尔爱尔兰有限公司和日本神经科学学会。版权所有。
The novel technology of induced neuronal cells (iN cells) is promising for translational neuroscience, as it allows the conversion of human fibroblasts into cells with postmitotic neuronal traits. However, a major technical barrier is the low conversion rate. To overcome this problem, we optimized the conversion media. Using our improved formulation, we studied how major mental illness-associated chromosomal abnormalities may impact the characteristics of iN cells. We demonstrated that our new iN cell culture protocol enabled us to obtain more precise measurement of neuronal cellular phenotypes than previous iN cell methods. Thus, this iN cell culture provides a platform to efficiently obtain possible cellular phenotypes caused by genetic differences, which can be more thoroughly studied in research using other human cell models such as induced pluripotent stem cells. (C) 2015 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.