Ontogenesis of tyrosine hydroxylase-immunopositive structures in the rat hypothalamus. An atlas of neuronal cell bodies
Ontogenesis of tyrosine hydroxylase-immunopositive structures in the rat hypothalamus. An atlas of neuronal cell bodies
复制标题
大鼠下丘脑酪氨酸羟化酶免疫阳性结构的个体发生。
DOI：
10.1016/0306-4522(89)90338-2
复制
发表时间：
1989
期刊：
影响因子：
3.3
通讯作者：
M. S. Mitskevich
中科院分区：
文献类型：
--
作者：
M. Ugrumov;J. Taxi;A. Tixier;J. Thibault;M. S. Mitskevich
本文研究了大鼠从胎龄第12天至生后第9天下丘脑和隔区儿茶酚胺能系统的发育。使用抗血清酪氨酸羟化酶的预包埋免疫细胞化学可视化的儿茶酚胺能结构。此外，使用银和金强化二氨基联苯胺产物，以使免疫细胞化学技术更敏感。在本文中，只有酪氨酸羟化酶免疫阳性神经元（胞体）的外观和分布的数据，而下丘脑与酪氨酸羟化酶免疫阳性纤维的儿茶酚胺能神经支配是一个附带的文件的主题。胎龄13天，在下丘脑和隔区的原基中首次观察到稀疏的酪氨酸羟化酶免疫阳性神经元。它们的数量随着年龄的增长而逐渐增加，到胎龄第15天，它们已经产生了一个大的背侧积累。从胎龄18天开始，酪氨酸羟化酶免疫阳性神经元开始占据其确定的位置，主要集中在下丘脑：在齿状回，室周和弓状核。在较小程度上，它们集中在内侧视前区，视交叉上，视上，室旁核，背内侧和下丘脑前核。酪氨酸羟化酶免疫阳性神经元在下丘脑和隔区在个体发育过程中的分布的数据进行了总结，在精确atlas.Primestival小双和单极的儿茶酚胺能神经元首先观察到的最年轻的胎儿在个体发育过程中进行细胞分化，从而产生至少两个不同的人口定位腹侧，主要是在弓状核和背侧，在inclinerta。前者的神经元仍然与最年轻的胎儿相似，而后者的神经元的大小显着增加，形成几个长的，高度分叉的过程。
The development of the catecholaminergic system in the hypothalamus and in the septal region was studied in rats from the 12th fetal day until the 9th postnatal day. Catecholaminergic structures were visualized with pre-embedding immunocytochemistry using antiserum to tyrosine hydroxylase. An intensification of diaminobenzidine product with silver and gold was additionally applied to make the immunocytochemical technique more sensitive. In this paper only the data on the appearance and distribution of the tyrosine hydroxylase-immunopositive neurons (cell bodies) are presented, whereas the catecholaminergic innervation of the hypothalamus with the tyrosine hydroxylase-immunopositive fibers is the topic of an accompanying paper. Sparse tyrosine hydroxylase-immunopositive neurons were first observed in the anlage of the hypothalamus and septal region on the 13th fetal day. Their number increased progressively with age and by the 15th fetal day they already gave rise to a large dorsal accumulation. From the 18th fetal day on, tyrosine hydroxylase immunopositive neurons began to occupy their definitive positions, mainly concentrating within the hypothalamus: in the zona incerta, periventricular and arcuate nuclei. To a lesser extent, they were concentrated in the medial preoptic area, suprachiasmatic, supraoptic, paraventricular, dorsomedial and anterior hypothalamic nuclei. The data on the distribution of the tyrosine hydroxylase-immunopositive neurons both in the hypothalamus and in the septal region during ontogenesis are summarized in the precise atlas.Primarily small bi- and unipolar catecholaminergic neurons first observed in the youngest fetuses undergo cytodifferentiation during ontogenesis, giving rise to at least two different populations localized ventrally, mainly in the arcuate nucleus and dorsally, in the zona incerta. The neurons of the former population remain similar to those of the youngest fetuses, whereas the neurons of the latter increase significantly in size, forming several long, highly ramified processes.