Clonal analysis of quail neural crest cells: they are pluripotent and differentiate in vitro in the absence of noncrest cells.
Clonal analysis of quail neural crest cells: they are pluripotent and differentiate in vitro in the absence of noncrest cells.
复制标题
鹌鹑神经嵴细胞的克隆分析：它们是多能的，并且在没有非嵴细胞的情况下在体外分化。
DOI：
10.1016/0012-1606(80)90501-1
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发表时间：
1980
影响因子：
2.7
通讯作者：
A. M. Cohen
中科院分区：
文献类型：
--
作者：
M. Sieber;A. M. Cohen
为了确定神经嵴细胞是否是多能性的，并建立是否分化发生在noncrest细胞的情况下，设计了一种细胞培养方法，其中分化可以在来自单个分离的神经嵴细胞的克隆中进行检查。单个神经嵴细胞，这是分离发病ofinvivomigration之前，产生了三种类型的克隆：色素，无色素，和混合。色素克隆仅由黑素细胞组成，而混合和无色素克隆中的一些无色素细胞含有儿茶酚胺，将其鉴定为肾上腺素能细胞。来自鹌鹑体节或鸡皮肤成纤维细胞培养物的细胞外基质刺激肾上腺素能分化和轴突形成。这些结果表明，第一次的存在多能鹌鹑神经嵴细胞，产生至少两个后代，黑素细胞和神经元细胞。他们还表明，连续的直接相互作用与noncrest细胞不需要这两种细胞类型的分化。然而，来自非嵴细胞的细胞外基质成分可能在肾上腺素能表型的表达中起重要作用。
To determine if neural crest cells are pluripotent and establish whether differentiation occurs in the absence of noncrest cells, a cell culture method was devised in which differentiation could be examined in clones derived from single, isolated neural crest cells. Single neural crest cells, which were isolated before the onset ofin vivomigration, gave rise to three types of clones: pigmented, unpigmented, and mixed. Pigmented clones consisted of melanocytes only, whereas some unpigmented cells in mixed and unpigmented clones contained catecholamines, identifying them as adrenergic cells. Extracellular matrix derived from quail somite or chick skin fibroblast cultures stimulated adrenergic differentiation and axon formation. These results demonstrate for the first time the existence of pluripotent quail neural crest cells that give rise to at least two progeny, melanocytes and neuronal cells. They also suggest that continuous direct interactions with noncrest cells are not required for the differentiation of these two cell types. However, components of the extracellular matrix derived from noncrest cells may play an important role in expression of the adrenergic phenotype.