Repetitive Indel Markers within the ALMT1 Gene Conditioning Aluminium Tolerance in Wheat (Triticum aestivum L.)
Repetitive Indel Markers within the ALMT1 Gene Conditioning Aluminium Tolerance in Wheat (Triticum aestivum L.)
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DOI：
10.1007/s11032-006-9025-2
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发表时间：
2006-08
影响因子：
3.1
通讯作者：
H. Raman;R. Raman;Rachel H. Wood;P. Martin
中科院分区：
文献类型：
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作者：
H. Raman;R. Raman;Rachel H. Wood;P. Martin
ALMT 1基因编码的膜蛋白，促进铝刺激苹果酸流出已被鉴定和定位在小麦（Triticum aestivumL.）。在此，我们已经确定了针对插入/缺失（indel）和SSR重复内的ALMT 1基因的内含子3区域的分子标记。两个标记：ALMT 1-SSR 3a和ALMT 1-SSR 3b在来自Diamondbird（耐铝）/Janz（铝敏感）的双单倍体群体中表现出与耐铝、苹果酸外排和区分ALMT 1 - 1和ALMT 1 - 2等位基因的CAPS标记的完全共分离。利用重复indel标记对20个不同小麦基因型进行亲本筛选，结果表明ALMT 1 SSR 3位点存在6个等位变异。序列分析证实，这些变异是由于插入缺失，SSR重复的拷贝数，和SSR重复内的碱基替换。内含子3的较高变异水平表明该基因组区域受到插入缺失、SSR和单核苷酸多态性的限制。结果表明，与耐铝基因座共分离的重复indel标记将有助于标记辅助选择和群体与进化研究。
ALMT1gene encoding a membrane protein that facilitates an aluminium stimulated malate efflux has been characterised and mapped in wheat (Triticum aestivumL.). Here, we have identified molecular markers targeting insertion/deletion (indel) and SSR repeats within intron 3 region of theALMT1gene. Both the markers: ALMT1-SSR3a and ALMT1-SSR3b based on repetitive indels, exhibited complete cosegregation with Al tolerance, malate efflux, and a CAPS marker discriminatingALMT1-1andALMT1-2alleles, in a doubled haploid population derived from Diamondbird (Al-tolerant)/Janz (Al-sensitive). A parental screen of 20 diverse wheat genotypes with repetitive indel markers indicated that six allele variants exist at theALMT1SSR3locus. Sequence analysis confirmed that these variations were due to indels, copy number of SSR repeats, and base substitution within SSR repeats. The higher level of variation in intron 3 suggests that this genomic region has been constrained by indels, SSR and single nucleotide polymorphisms. Results have proven that repetitive indel markers cosegregating with the Al tolerance locus will be useful for marker assisted selection and population and evolution studies.