GENETIC AND PHYSICAL MAPPING OF THE NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN-1 (NRAMP1) IN CHICKEN
GENETIC AND PHYSICAL MAPPING OF THE NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN-1 (NRAMP1) IN CHICKEN
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DOI：
10.1007/bf00539010
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发表时间：
1995-11-01
期刊：
影响因子：
2.5
通讯作者：
MALO, D
中科院分区：
文献类型：
--
作者：
HU, J;BUMSTEAD, N;MALO, D
利用连锁分析和荧光原位杂交技术，对鸡天然抗性相关巨噬细胞蛋白1（NRAMP 1）基因进行了定位。小鼠Nramp1基因的鸡同源物被定位到位于染色体（Chr）7q13上的连锁群，该连锁群包括先前已被定位到小鼠Chr 1或人类Chr 2q的三个基因（CD28、NDUSF1和EF1B）。脉冲场凝胶电泳的物理图谱显示，NRAMP 1与绒毛蛋白基因紧密相连，携带NRAMP 1的染色体区域的序列（基因顺序和CpG岛的存在）在鸡和哺乳动物基因组之间是很保守的。小鼠Chr 1，人Chr 2q，鸡Chr 7q包含NRAMP 1，代表哺乳动物和鸟类基因组之间的大的保守染色体片段。鸡NRAMP1基因的染色体定位是确定其在该物种对沙门氏菌病的不同易感性中可能作用的第一步。
The chicken natural resistance-associated macrophage protein 1 (NRAMP1) gene has been mapped by linkage analysis by use of a reference panel to develop the chicken molecular genetic linkage map and by fluorescence in situ hybridization. The chicken homolog of the murine Nramp1 gene was mapped to a linkage group located on Chromosome (Chr) 7q13, which includes three genes (CD28, NDUSF1, and EF1B) that have previously been mapped either to mouse Chr 1 or to human Chr 2q. Physical mapping by pulsed-field gel electrophoresis revealed that NRAMP1 is tightly linked to the villin gene and that the genomic organization (gene order and presence of CpG islands) of the chromosomal region carrying NRAMP1 is well conserved between the chicken and mammalian genomes, The regions on mouse Chr 1, human Chr 2q, and chicken Chr 7q that encompass NRAMP1 represent large conserved chromosomal segments between the mammalian and avian genomes. The chromosome mapping of the chicken NRAMP1 gene is a first step in determining its possible role in differential susceptibility to salmonellosis in this species.