Impact of Turnip yellows virus infection on seed yield of an open-pollinated and hybrid canola cultivar when inoculated at different growth stages
Impact of Turnip yellows virus infection on seed yield of an open-pollinated and hybrid canola cultivar when inoculated at different growth stages
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DOI：
10.1016/j.virusres.2019.197847
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发表时间：
2020-02-01
期刊：
影响因子：
5
通讯作者：
Coutts, B. A.
中科院分区：
文献类型：
--
作者：
Congdon, B. S.;Baulch, J. R.;Coutts, B. A.
芜菁黄化病毒(TuYV；Luteoviridae，PoleroVirus)是侵染澳大利亚西南部谷物带油菜(Brassica Napus)的最具经济破坏性的病毒。然而，不同生长阶段的TuYV感染对油菜种子产量的影响尚未得到检验。这些信息对于实施有针对性的管理战略至关重要。通过4个温室试验，研究了油菜自由授粉品种(ATR Bonito)和杂交油菜(Hyola(R)404RR)在GS12(两叶展开)、GS17(七叶展开)、GS30(茎伸长开始)和GS65(盛花期)接种TuYV后的种子产量损失。在GS12和GS17接种时，cv.与健康植物相比，鳄鱼分别遭受了30%和36%的种子产量损失。同样，cv.404RR在GS12和GS17分别遭受41%和26%的种子产量损失。然而，在GS30接种时，当cv.鳄鱼植株遭受26%的种子产量损失。404RR没有发生重大损失。两个品种均未因接种GS65而遭受种子产量损失。从这些实验中收集了更多的信息，以改进采样方案，以加强分子和血清学技术对TuYV的检测。当油菜植株处于开花前生长阶段时，在接种后7-14天(DAI)，在最年轻的叶片上可靠地检测到TuYV。一旦开始开花，在花瓣和花蕾中持续检测到7-14个DAI的TuYV。相比之下，无论生长阶段如何，测试最老的叶片通常会导致延迟检测或假阴性。这项研究产生的信息有助于量化旨在防止TuYV在开花前油菜作物中传播的管理战略的价值，并最终提高资源利用效率。
Turnip yellows virus (TuYV; family Luteoviridae, genus Polerovirus) is the most economically damaging virus infecting canola (Brassica napus) in the south-west Australian grainbelt. However, the impact of TuYV infection at different growth stages on canola seed yield has not been examined. This information is vital for implementing targeted management strategies. Four glasshouse experiments were conducted to examine seed yield losses incurred by an open-pollinated (ATR Bonito) and hybrid (Hyola (R) 404RR) canola cultivar when aphid-inoculated with TuYV at GS12 (two leaves unfolded), GS17 (seven leaves unfolded), GS30 (beginning of stem elongation) and GS65 (full flowering). When inoculated at GS12 and GS17, cv. Bonito plants incurred 30 % and 36 % seed yield losses, respectively, compared to healthy plants. Similarly, cv. 404RR incurred 41 % and 26 % seed yield losses at GS12 and GS17, respectively. However, when inoculated at GS30, whilst cv. Bonito plants incurred a 26 % seed yield loss, cv. 404RR incurred no significant loss. Neither cultivar incurred seed yield losses from inoculation at GS65. Additional information was collected from these experiments to improve sampling protocols to enhance TuYV detection, with a molecular and serological technique. When canola plants were at pre-flowering growth stages, TuYV was reliably detected 7-14 days after inoculation (DAI) in the youngest leaf. Once flowering had begun, TuYV was consistently detected 7-14 DAI in petals and flower buds. In contrast, regardless of growth stage, testing the oldest leaf regularly resulted in delayed detection or false negatives. Information generated in this study helps to quantify the value of management strategies targeted at preventing TuYV spread in pre-flowering canola crops and ultimately increase the efficiency of resource use.