Buoyancy Limitation of Filamentous Cyanobacteria under Prolonged Pressure due to the Gas Vesicles Collapse
Buoyancy Limitation of Filamentous Cyanobacteria under Prolonged Pressure due to the Gas Vesicles Collapse
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DOI：
10.1007/s00267-017-0875-7
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发表时间：
2017-08-01
影响因子：
3.5
通讯作者：
Kaneko, Yasuko
中科院分区：
文献类型：
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作者：
Lakmali Abeynayaka, Helayaye Damitha;Asaeda, Takashi;Kaneko, Yasuko
淡水蓝细菌 Pseudanabaena Galeata 在人工产生的压力下培养，该压力相当于深水的静水压力。在不同时间间隔（5 分钟、1 天和 5 天）分析这些条件下气泡体积的变化和细胞的浮力状态。通过透射电子显微镜图像观察细胞气泡形态的变化。借助改进的光学显微镜观察控制浮力的细胞的沉降速度（Vs）。此外，还检查了长时间压力对细胞镇流器成分（蛋白质和多糖）的影响。升高的压力条件减少了细胞镇流器并导致 Pseudanabaena galeata 细胞中的气泡完全消失。因此，蓝藻细胞在研究期间不能漂浮。该研究的观察和结果表明，在湖泊低潮中自然发生的静水压力可以潜在地应用于抑制蓝藻细胞的重新悬浮。
Freshwater cyanobacterium Pseudanabaena galeata were cultured in chambers under artificially generated pressures, which correspond to the hydrostatic pressures at deep water. Variations occurred in gas vesicles volume, and buoyancy state of cells under those conditions were analyzed at different time intervals (5 min, 1 day, and 5 days). Variations in gas vesicles morphology of cells were observed by transmission electron microscopy images. Settling velocity (Vs) of cells which governs the buoyancy was observed with the aid of a modified optical microscope. Moreover, effects of the prolonged pressure on cell ballast composition (protein and polysaccharides) were examined. Elevated pressure conditions reduced the cell ballast and caused a complete disappearance of gas vesicles in Pseudanabaena galeata cells. Hence cyanobacteria cells were not able to float within the study period. Observations and findings of the study indicate the potential application of hydrostatic pressure, which naturally occurred in hypolimnion of lakes, to inhibit the re-suspension of cyanobacteria cells.