Quantification of small molecule organic acids from Mycobacterium tuberculosis culture supernatant using ion exclusion liquid chromatography/mass spectrometry.
Quantification of small molecule organic acids from Mycobacterium tuberculosis culture supernatant using ion exclusion liquid chromatography/mass spectrometry.
复制标题
使用离子排阻液相色谱/质谱法对结核分枝杆菌培养物上清液中的小分子有机酸进行定量。
DOI：
10.1002/rcm.2745
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Dowd,CynthiaS
中科院分区：
文献类型：
--
作者：
Goodwin,MichaelB;Boshoff,HelenaI;Barry3rd,CliftonE;Dowd,CynthiaS
Quantitative analysis of cellular small molecule organic acids of intermediary metabolism can provide critical insight into bacterial metabolic pathways. The concentration of these metabolites in culture supernatant varies at different growth stages or under particular environmental conditions reflecting both the energy and the biosynthetic needs, yielding metabolic information about the microorganism. The method described here utilizes ion exclusion chromatography with formic acid, coupled with a mass‐selective detector using selective ion monitoring with negative mode electrospray ionization (SIM ES−), to detect and quantify several small organic acids in culture supernatants. The µM limits of quantitation (LOQs) were found to be 5.5 ± 0.9 for pyruvate, 7.0 ± 0.4 for malate, 2.5 ± 0.5 for succinate, 12.7 ± 0.8 for lactate, and 6.6 ± 0.2 for fumarate. The method was used to detect and quantify these acids in the culture supernatants fromMycobacterium tuberculosis. Supernatant samples were spiked with stable‐isotope‐labeled internal standards, and the organic acids were quantified by isotope ratiometry. Published in 2006 by John Wiley & Sons, Ltd.
