Prolonged circulation of large polymeric nanoparticles by non-covalent adsorption on erythrocytes
Prolonged circulation of large polymeric nanoparticles by non-covalent adsorption on erythrocytes
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DOI：
10.1016/j.jconrel.2004.08.005
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发表时间：
2004-11-05
影响因子：
10.8
通讯作者：
Mitragotri, S
中科院分区：
文献类型：
--
作者：
Chambers, E;Mitragotri, S
聚合物纳米颗粒已被广泛研究用作血管内药物递送载体;然而，它们的应用受到网状内皮系统（RES）从循环中快速清除的限制。先前改善血管循环的尝试集中在使用聚合物如泊洛沙胺、泊洛沙姆和聚乙二醇进行表面改性以防止调理作用。我们报告了一种通过将纳米颗粒锚定在红细胞（RBC）表面来延长血管内颗粒循环的新方法。我们假设粘附在RBC上的颗粒可以逃避RES清除，这是由于RBC的这种能力。这种方法的动机是某些细菌所采取的策略，例如，血巴尔通体，粘附在RBC上并在循环中保持数周。在RBC上吸附后，观察到大至450 nm的纳米颗粒的延长循环。尽管颗粒最终从循环中消除，但RBC未被清除。RBC锚定纳米颗粒为血管内药物输送和血池成像提供了一种新方法。(C)2004 Elsevier B. V.保留所有权利。
Polymeric nanoparticles have been extensively studied for use as intravascular drug delivery vehicles; however, their applications are limited by rapid clearance from circulation by the reticuloendothelial system (RES). Previous attempts to improve vascular circulation have focused on surface modification using polymers such as poloxamines, poloxamers, and polyethylene glycol, to prevent opsononization. We report on a novel method of prolonging intravascular particle circulation by anchoring the nanoparticles to the surface of red blood cells (RBCs). We hypothesize that particles adhered to RBCs can escape RES clearance due to the ability of RBCs to do so. This method is motivated by the strategy adopted by certain bacteria, for example, hemobartonella, that adhere to RBCs and remain in circulation for several weeks. Prolonged circulation of nanoparticles as large as 450 nm was observed after adsorption on RBCs. Although particles were eventually eliminated from circulation, RBCs were not cleared. RBC-anchored nanoparticles offer a novel approach for intravascular drug delivery and blood pool imaging. (C) 2004 Elsevier B.V. All rights reserved.