Alteration of salivary glycopatterns in oral lichen planus
Alteration of salivary glycopatterns in oral lichen planus
复制标题
口腔扁平苔藓唾液糖模式的改变
DOI：
10.1080/1354750x.2017.1405284
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发表时间：
2018-01-01
期刊：
影响因子：
2.6
通讯作者：
Chen, Lihua
中科院分区：
文献类型：
--
作者：
Fang, Liang;Liu, Qing;Chen, Lihua
背景：口腔扁平苔藓（OLP）是最常见的口腔粘膜病变之一，影响0.5-2%的成年人。OLP与其他口腔粘膜疾病很难区分。唾液蛋白聚糖的结构变化可能是OLP的可靠指标。目的：研究口腔扁平苔藓（OLP）患者唾液蛋白糖基化水平的变化。材料与方法：选择28例OLP患者和30例年龄、性别匹配的健康志愿者作为实验组，采用凝集素基因芯片技术检测唾液蛋白糖基化水平的差异。结果：3种凝集素[Aleuria aurantia lectin（AAL）、Phytolacca americana（PWM）、Phaseolus vulgaris agglutinin（E+L）、（PHA-E+L）]识别的糖蛋白在OLP唾液中主要增加。同时，这些糖蛋白也表现出显着的年龄相关alternations.Conclusions：这项研究提供了一个新的基本洞察唾液糖蛋白在OLP，并有助于开发新的潜在的生物标志物诊断OLP。
Background: Oral lichen planus (OLP) is one of the most common oral mucosal lesions affecting 0.5-2% of the adult population. It is difficult to distinguish between OLP and other oral mucosal diseases. Structural changes in the glycans of saliva proteins might be reliable indicators of OLP. However, little is known about the alteration of salivary glycopatterns during OLP.Objective: We aimed to investigate the alterations of salivary protein glycosylation related to OLP.Material and methods: Twenty-eight patients with OLP and 30 age- and sex-matched healthy volunteers (HVs) were enrolled in the test group to probe the difference of salivary glycopatterns using lectin microarrays. The lectin blotting were further utilized to validate the expression of certain glycans.Results: The glycoproteins recognized by three lectins [Aleuria aurantia lectin (AAL); Phytolacca americana (PWM); Phaseolus vulgaris agglutinin (E+L), (PHA-E+L)] were mainly increasing in the saliva of OLP. Meanwhile, these glycoproteins also exhibited significant age-associated alterations.Conclusions: This study provided a new basic insight into salivary glycopatterns in OLP and helped to develop new potential biomarkers for diagnosis of OLP.