Anatomic heterogeneity of vascular aging - Role of nitric oxide and endothelin
Anatomic heterogeneity of vascular aging - Role of nitric oxide and endothelin
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DOI：
10.1161/01.hyp.30.4.817
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发表时间：
1997-10-01
期刊：
影响因子：
8.3
通讯作者：
Luscher, TF
中科院分区：
文献类型：
--
作者：
Barton, M;Cosentino, F;Luscher, TF
我们研究了年龄，心血管危险因素，血管功能方面的内皮型一氧化氮合酶（eNOS），超氧化物歧化酶（SOD），内皮素（ET-1）在主动脉和股动脉的大鼠。获得了对乙酰胆碱、钙离子载体A23187、去甲肾上腺素、ET-1、大内皮素、硝普钠和外源性SOD的浓度响应曲线。采用逆转录聚合酶链反应（RT-PCR）检测eNOS mRNA表达，细胞色素c荧光法检测SOD活性，放射免疫法检测ET-1血浆浓度。老年大鼠主动脉对乙酰胆碱和钙离子载体A23157的舒张作用、基础NO释放和内皮细胞eNOS mRNA表达均降低（P
We investigated the effects of aging, a cardiovascular risk factor, on vascular function with regard to endothelial nitric oxide synthase (eNOS), superoxide dismutase (SOD), and endothelin (ET-1) in aorta and femoral artery of the rat. Concentration-response curves to acetylcholine, calcium ionophore A23187, norepinephrine, ET-1, big endothelin, sodium nitroprusside, and exogenous SOD were obtained. Expression of eNOS mRNA was analyzed by reverse-transcription polymerase chain reaction, SOD activity was assessed using a chemiluminescence-based cytochrome c assay, and ET-1 plasma concentrations were measured by radioimmunoassay. In aorta of old rats, relaxations to acetylcholine and calcium-ionophore A23157, basal NO release, and expression of eNOS mRNA in aortic endothelial cells were reduced (P