CONTRACTION OF ISOLATED BRUSH-BORDERS FROM INTESTINAL EPITHELIUM
CONTRACTION OF ISOLATED BRUSH-BORDERS FROM INTESTINAL EPITHELIUM
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DOI：
10.1083/jcb.70.3.541
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发表时间：
1976-01-01
影响因子：
7.8
通讯作者：
KARNOVSKY, MJ
中科院分区：
文献类型：
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作者：
RODEWALD, R;NEWMAN, SB;KARNOVSKY, MJ
从新生大鼠小肠上皮细胞分离的刷状缘能够在ATP和Mg 2+存在下收缩;不需要Ca 2+。收缩的特征是粘附小带区域的质膜收缩，随后刷状缘变圆。未观察到微绒毛的移动或一致缩短。收缩似乎涉及5 - 7-nm直径的微丝在终端网与小带adherens。这些丝与重的解肌球蛋白结合，就像微绒毛的肌动蛋白核心丝一样。收缩的模型中，在完整的细胞中，终端网丝和核心丝相互作用，产生缩短的微绒毛。
Brush borders isolated from epithelial cells from the small intestine of neonatal rats are able to contract in the presence of ATP and Mg2+; Ca2+ is not required. Contraction is characterized by a pinching-in of the plasma membrane in the region of the zonula adherens and a subsequent rounding of the brush borders. No movement or consistent shortening of the microvilli is observed. The contraction appears to involve the 5- to 7-nm diameter microfilaments in the terminal web which associate with zonula adherens. These filaments bind heavy meromyosin as do the actin core filaments of the microvilli. A model for contraction is presented in which, in the intact cell, terminal web filaments and core filaments interact to produce shortening of the microvilli.