Distinct and direct synergistic effects of IL-1 and IL-6 on proliferation and differentiation of primitive murine hematopoietic progenitor cells in vitro.
Distinct and direct synergistic effects of IL-1 and IL-6 on proliferation and differentiation of primitive murine hematopoietic progenitor cells in vitro.
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DOI：
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发表时间：
1994-10
影响因子：
2.6
通讯作者：
S. Jacobsen;F. Ruscetti;C. Okkenhaug;Eli Lien;M. Ortiz;O. P. Veiby;J. Keller
中科院分区：
文献类型：
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作者：
S. Jacobsen;F. Ruscetti;C. Okkenhaug;Eli Lien;M. Ortiz;O. P. Veiby;J. Keller
虽然已证明白细胞介素-1（IL-1）和IL-6与集落刺激因子（CSF）和干细胞因子（SCF）协同刺激原始造血祖细胞的髓样集落形成，但尚未确定这些作用是否是直接介导的。在本研究中，IL-1和IL-6对单独培养和接种的原始Lin-Sca-1+小鼠骨髓祖细胞的直接作用进行了研究。IL-1和IL-6不仅表现出重叠，而且表现出不同的直接协同作用模式。虽然IL-1或IL-6作为单一生长因子没有增殖作用，但IL-1与粒细胞-巨噬细胞CSF（GM-CSF）、IL-3、CSF-1和SCF（但不与粒细胞-CSF（G-CSF）组合）将Lin-Sca-1+祖细胞的克隆频率提高了3至5倍，而IL-6响应于所有4种CSF和SCF将克隆频率提高了2至7倍。在所有情况下，观察到的集落大小也增加。此外，IL-1和IL-6的联合作用导致CSF和SCF刺激的Lin-Sca-1+高增殖潜能集落形成细胞（HPP-CFC）的集落形成的相加或协同增强。最后，IL-6，而不是IL-1，增加了CSF和SCF刺激培养物中观察到的未成熟母细胞的数量。
While interleukin-1 (IL-1) and IL-6 have been demonstrated to synergize with colony-stimulating factors (CSFs) and stem cell factor (SCF) to stimulate myeloid colony formation of primitive hematopoietic progenitor cells, it has not yet been established whether these effects are directly mediated. In the present study, direct effects of IL-1 and IL-6 were examined on primitive Lin-Sca-1+ murine bone-marrow progenitor cells that were cultured and plated individually. IL-1 and IL-6 showed not only overlapping, but also distinct, patterns of direct synergy. While IL-1 or IL-6 had no proliferative effects as single growth factors, IL-1, in combination with granulocyte-macrophage CSF (GM-CSF), IL-3, CSF-1, and SCF, but not granulocyte-CSF (G-CSF), enhanced the cloning frequency of Lin-Sca-1+ progenitors three- to five-fold, whereas IL-6 increased the cloning frequency in response to all four CSFs and SCF two- to seven-fold. In all cases, the size of the colonies observed were increased as well. Furthermore, the combined action of IL-1 and IL-6 resulted in additive or synergistic enhancement of CSF- and SCF-stimulated colony formation of Lin-Sca-1+ high proliferative potential colony-forming cells (HPP-CFCs). Finally, IL-6, but not IL-1, enhanced the number of immature blast cells observed in CSF- and SCF-stimulated cultures.