First domain encoding sequence mediates human class II beta-chain gene cross-hybridization.
First domain encoding sequence mediates human class II beta-chain gene cross-hybridization.
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第一结构域编码序列介导人类II类β链基因交叉杂交。
DOI：
10.1007/bf00375859
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发表时间：
1988
期刊：
影响因子：
3.2
通讯作者：
Trucco,M
中科院分区：
文献类型：
--
作者：
Turco,E;Fritsch,R;Trucco,M
HLA II类分子是表面异源二聚体，其在免疫应答的起始中是必需的。由不同的II类分子表达的多态性的量在很大程度上取决于其β链的多态性结构。II类β基因之间的交叉杂交经常阻碍供体基因组DNA的限制性片段长度多态性分析。在这份报告中，我们表明，人类II类β基因之间的交叉杂交是由一个区域的高度同源性，丰富的C和G残基，之间的第一个结构域编码序列ofDP，DQ，和DR基因。从用作标记探针的片段中去除含有该区域的DNA片段，所述标记探针针对其它基因座处的基因的相应片段或针对内切核酸酶消化的基因组DNA，完全消除或大大减少了交叉杂交。此外，用缩短的探针产生的RFLP模式比用原始基因制成的探针产生的RFLP模式信息量更大，解释起来也简单得多。
HLA class II molecules are surface heterodimers which are essential in the initiation of immune responses. The amount of polymorphism expressed by the different class II molecules is largely dependent on the polymorphic structure of their beta chains. Cross-hybridization between, class II beta genes frequently hampered restriction fragment length polymorphism analysis of donor genomic DNA. In this report we show that the cross-hybridization between human class II beta genes is mediated by a region of high homology, rich in C and G residues, between the first domain encoding sequences ofDP, DQ, andDRgenes. The removal of the DNA segment containing this region from the fragments used as labeled probes against the corresponding fragments of the genes at other loci or against endonuclease digested genomic DNA completely eliminated or drastically reduced the cross-hybridization. Also, the RFLP patterns generated with the shortened probes were more informative and much simpler to interpret than were these generated with probes made from the original genes.