The anti-phospholipid antibody-dependent and independent effects of periodontopathic bacteria on threatened preterm labor and preterm birth
The anti-phospholipid antibody-dependent and independent effects of periodontopathic bacteria on threatened preterm labor and preterm birth
复制标题
DOI：
10.1007/s00404-013-2741-z
复制
发表时间：
2013-02
影响因子：
2.6
通讯作者：
C. Ye;Sayaka Katagiri;N. Miyasaka;Pariksha Bharti;H. Kobayashi;Y. Takeuchi;Yoshihito Momohara;M. Sekiguchi;Satoko Takamine;T. Nagasawa;Y. Izumi
中科院分区：
文献类型：
--
作者：
C. Ye;Sayaka Katagiri;N. Miyasaka;Pariksha Bharti;H. Kobayashi;Y. Takeuchi;Yoshihito Momohara;M. Sekiguchi;Satoko Takamine;T. Nagasawa;Y. Izumi
目的牙周病被认为是先兆早产（TPL）和早产（PB）的危险因素，但其致病机制尚未阐明。我们假设牙周病细菌感染可能通过TLRVYK肽对β-2糖蛋白I（抗磷脂综合征的靶分子）的分子模拟而增强血栓形成。本研究旨在探讨牙周炎对TPL和PB的影响。MethodsNineteent-five孕妇（47 TPL和48名健康受试者）参加。检查牙周炎临床参数和牙周致病菌。采用兔多克隆抗体（SIRVYK对伴放线菌聚集菌、TLRIYT对牙龈卟啉单胞菌、TLALYK对齿垢密螺旋体），通过酶联免疫吸附试验（ELISA）检测TLRVYK肽与牙周致病菌上同源肽的分子模拟。ELISA法检测血清超敏C反应蛋白、抗TLRVYK和抗SIRVYK IgG抗体。结果在特异性抗细菌同源肽的兔抗体中，只有抗SIRVYK IgG抗体与TLRVYK肽发生反应。多因素分析显示抗SIRVYK IgG抗体与TPL的诊断显著相关。在95例分娩中，14例（14.7%）早产。抗SIRVYK IgG抗体>中位数组的早产率高于≤中位数组。47例TPL患者中13例有PB，有序Logistic回归分析显示既往吸烟、有无P.牙龈炎、抗SIRVYK IgG抗体与PB呈显著相关。对SIRVYK的抗体应答可能与TPL和PB有关。
PurposePeriodontal disease is considered to be a risk factor for threatened preterm labor (TPL) and preterm birth (PB), but pathogenic mechanisms have not yet been elucidated. We hypothesized that infection with periodontopathic bacteria may enhance thrombosis through molecular mimicry with TLRVYK peptides on beta-2 glycoprotein I, a target molecule in anti-phospholipid syndrome. This study aimed to examine the effects of periodontitis on TPL and PB.MethodsNinety-five pregnant women (47 TPL and 48 healthy subjects) participated. Periodontal clinical parameters and periodontopathic bacteria were examined. Molecular mimicry between TLRVYK peptides and homologous peptides on the periodontopathic bacteria was examined by enzyme-linked immunosorbent assay (ELISA) using rabbit polyclonal antibodies specific for the respective peptides (SIRVYK onAggregatibacter actinomycetemcomitans, TLRIYT onPorphyromonus gingivalis, and TLALYK onTreponema denticola). Serum high-sensitivity C-reactive protein, anti-TLRVYK and anti-SIRVYK IgG antibodies were measured using ELISA.ResultsAmong the rabbit antibodies specific for the bacterial homologous peptides, only anti-SIRVYK IgG antibody reacted with TLRVYK peptides. Multivariable analysis showed that anti-SIRVYK IgG antibody was significantly associated with diagnosis of TPL. Of 95 births, 14 (14.7 %) delivered preterm. The preterm birth rate was higher in the anti-SIRVYK IgG antibody >median group than in the ≤median group. Of the 47 TPL subjects 13 had PB, and ordinal logistic regression analysis revealed that past smoking, presence ofP. gingivalisand anti-SIRVYK IgG antibody were significantly correlated with PB.ConclusionsInfection withP. gingivalisand the antibody response to SIRVYK might be associated with TPL and PB.