Application of fluorescence correlation spectroscopy for velocity imaging in microfluidic devices
Application of fluorescence correlation spectroscopy for velocity imaging in microfluidic devices
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DOI：
10.1366/0003702042335957
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发表时间：
2004-10-01
影响因子：
3.5
通讯作者：
Weston, KD
中科院分区：
文献类型：
--
作者：
Kuricheti, KK;Buschmann, V;Weston, KD
在这篇文章中，我们提出并演示了一种利用共焦显微镜结合荧光相关光谱(FCS)绘制亚微米分辨率微流控系统中流体流量的技术。使用荧光聚合物纳米球作为流体运动示踪剂，可以记录到从50微米/S到大约10厘米/S的流速。给出并分析了聚二甲基硅氧烷玻璃微通道中流动的速度分布和图像。利用该方法，可以获得微器件内水平(俯视)面和垂直面上的速度图像。据我们所知，这是FCS制作速度图的第一份报告。高分辨率速度图可用于表征和优化微器件性能，并验证模拟工作。
In this paper we present and demonstrate a technique for mapping fluid flow rates in microfluidic systems with sub-micrometer resolution using confocal microscopy in conjunction with fluorescence correlation spectroscopy (FCS). Flow velocities ranging from similar to50 mum/s to similar to10 cm/s can be recorded using fluorescent polymer nanospheres as fluid motion tracers. Velocity profiles and images of the flow in poly(dimethylsiloxane)-glass microchannels are presented and analyzed. Using the method, velocity images along the horizontal (top view) and vertical planes within a microdevice can be obtained. This is, to our knowledge, the first report of FCS for producing velocity maps. The high-resolution velocity maps can be used to characterize and optimize microdevice performance and to validate simulation efforts.