NUCLEOTIDE-SEQUENCE OF AKV MURINE LEUKEMIA-VIRUS
NUCLEOTIDE-SEQUENCE OF AKV MURINE LEUKEMIA-VIRUS
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DOI：
10.1128/jvi.49.2.471-478.1984
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发表时间：
1984-01-01
影响因子：
5.4
通讯作者：
HERR, W
中科院分区：
文献类型：
--
作者：
HERR, W
AKV是一种内源性嗜亲性鼠白血病病毒，是白血病前期AKR小鼠中出现的重组致癌貂细胞病灶形成病毒的亲本之一。本文报道了从感染性分子克隆AKR-623中确定的AKV的8374个核苷酸长的序列。AKV的5“-前导序列延伸至核苷酸639，其后是编码gag和pol基因产物的长开放阅读框。阅读框被分开gag和pol基因的单个琥珀密码子中断。pol基因在AKV基因组的3“区域内与env基因重叠。AKV的5“区的核苷酸序列揭示了以下特征。5“-前导序列缺乏任何AUG密码子来启动gPr 80 gag的翻译，表明gPr 80 gag对于AKV的复制不是必需的。前导区的一小部分在序列上与密切相关的莫洛尼鼠白血病病毒不同，似乎与真核基因组中高度重复的序列有关。与莫洛尼鼠白血病病毒一样，在琥珀密码子的侧翼有一个潜在的RNA二级结构，该密码子将gag和pol基因分开。这种结构可能作为一个调节蛋白结合位点，控制gag和pol前体合成的相对水平。将AKV的3“区域的核苷酸序列与先前报道的来自AKV的感染性和非感染性分子克隆的序列进行比较。
AKV is an endogenous, ecotropic murine leukemia virus that serves as one of the parents of the recombinant, oncogenic mink cell focus-forming viruses that arise in preleukemic AKR mice. The 8374-nucleotide-long sequence of AKV, as determined from the infecitous molecular clone AKR-623, is reported. The 5''-leader sequence of AKV extends to nucleotide 639, after which lies a long open reading frame encoding the gag and pol gene products. The reading frame is interrupted by a single amber codon separating the gag and pol genes. The pol gene overlaps the env gene within the 3'' region of the AKV genome. The nucleotide sequence of the 5'' region of AKV reveals the following features. The 5''-leader sequence lacks any AUG codon to initiate translation of gPr80gag, suggesting that gPr80gag is not required for the replication of AKV. A short portion of the leader region diverges in sequence from the closely related Moloney murine leukemia virus and appears to be related to a sequence highly repeated in eukaryotic genomes. As in Moloney murine leukemia virus, there is a potential RNA secondary structure flanking the amber codon that separates the gag and pol genes. This structure might function as a regulatory protein binding site that controls the relative levels of synthesis of the gag and pol precursors. The nucleotide sequence of the 3'' region of AKV is compared with sequences reported previously from both infectious and noninfectious molecular clones of AKV.