Monoclonal antibodies induce the translocation, patching, and shedding of surface antigens of sea urchin spermatozoa.
Monoclonal antibodies induce the translocation, patching, and shedding of surface antigens of sea urchin spermatozoa.
复制标题
单克隆抗体诱导海胆精子表面抗原的易位、修补和脱落。
DOI：
10.1016/0014-4827(88)90253-4
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发表时间：
1988
影响因子：
3.7
通讯作者：
Vacquier,VD
中科院分区：
文献类型：
--
作者：
Trimmer,JS;Vacquier,VD
与海胆精子质膜蛋白外区结合的单抗可引起精子的可逆性聚集。解聚速率与温度有关，表明膜流动性参与其中。免疫荧光显示，解聚与表面结合的抗体进入大的聚集体，然后从细胞表面脱落的运动在时间上相关。对脱落抗原的电泳分析表明，它们与分离的精子膜蛋白的总组成相似。表面结合配体的移位和脱落可能是真核生物鞭毛膜的共同特征。
Monoclonal antibodies reacting with external domains of plasma membrane proteins of sea urchin spermatozoa cause the reversible aggregation of sperm. The rate of disaggregation is temperature dependent indicating the involvement of membrane fluidity. Immunofluorescence shows that disaggregation is temporally correlated with the movement of surface-bound antibody into large aggregates which are then shed from the cell surface. Electrophoretic analysis of the shed antigens shows that they resemble the total complement of proteins of isolated sperm membranes. The translocation and shedding of surface-bound ligands may be a common property of eukaryotic flagellar membranes.