Glycol methacrylate: An alternative method for embedding subcutaneous implants
Glycol methacrylate: An alternative method for embedding subcutaneous implants
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DOI：
10.1097/00004770-200104000-00005
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发表时间：
2001-04-01
影响因子：
4.2
通讯作者：
Souza, FJ
中科院分区：
文献类型：
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作者：
Gomes, JE;Gomes, BPFA;Souza, FJ
本研究的目的是研究使用甲基丙烯酸乙二醇酯 (GMA) 作为大鼠皮下组织的包埋材料，该组织接受带有牙髓封闭剂的管植入物。固定后，将标本在浓度高达 95% 的生长醇系列中脱水，浸入渗透 GMA 溶液中，然后浸入包埋溶液中。将块切成3.0μm的切片并用苏木精-伊红染色。细胞定义和染色的质量允许对渗透到管末端的细胞进行定量分析。还可以识别每种类型的炎症细胞。此外，可以清楚地区分慢性和急性炎症细胞。 GMA 技术易于执行且可重复，并且可以更好地定义组织细胞，从而可以定义炎症过程的程度。因此，它是评估牙髓封闭剂生物相容性的绝佳替代方案。
The aim of this investigation was to study the use of glycol methacrylate (GMA) as an embedding material for rat subcutaneous tissue, which received implants of tubes with endodontic sealer. After fixation, the specimens were dehydrated in a growing alcohol series up to 95%, immersed in infiltration GMA solution and then in embedding solution. The blocks were cut into 3.0 mum sections and stained with hematoxylin-eosin. The quality of cell definition and staining allowed a quantitative analysis of the cells infiltrated in the end of the tubes. It was also possible to identify each type of inflammatory cell. Moreover, it was possible to distinguish clearly chronic from acute inflammatory cells. The GMA technique is easy to execute and reproducible, and provides a better definition of tissue cells, thus permitting definition of the degree of the inflammatory process. Therefore, it is an excellent alternative for the evaluation of the biocompatibility of endodontic sealers.