SYNAPSE FORMATION DURING EMBRYOGENESIS ON GANGLION-CELLS LACKING A PERIPHERY
SYNAPSE FORMATION DURING EMBRYOGENESIS ON GANGLION-CELLS LACKING A PERIPHERY
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DOI：
10.1113/jphysiol.1974.sp010680
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发表时间：
1974-01-01
影响因子：
5.5
通讯作者：
PILAR, G
中科院分区：
文献类型：
--
作者：
LANDMESSER, L;PILAR, G
1.在鸡胚胎发育早期，将睫状神经节周边切除，研究其传递的发育。2.神经节中的外周剥夺神经元以正常数量分化并将功能性轴突送入节后神经。3.缺乏外周的神经节细胞遵循正常的发育顺序，在神经节细胞突触形成时发出短暂的树突，然后在出现萼时收回树突。4.突触在功能上和超微结构上都正常，在正常时间在所有神经节细胞上形成，并且传递正常，直到第34期。因此，神经节细胞突触的形成本身并不需要来自外周的信息。5.从第35阶段到第38阶段，大多数细胞死亡，因此只有8%的原始细胞保留在手术的神经节中。在这段时间内，许多细胞的传递都失败了，但在细胞损失之前只有很短的时间，所以传入神经阻滞可能不会导致细胞死亡。6.睫状体细胞和脉络膜细胞都实现了完全的细胞学分化，并且彼此不同，这表明这些细胞的独特特征的阐述不需要外周。突触前纤维也分化成典型的终扣和萼状终末。因此，节前末梢的类型并不依赖于神经节细胞建立适当的外周接触。7.目前还无法确定神经节细胞特异性是否受到外周的影响。8.外周移除影响神经节细胞迁移，从而形成两个神经节。大约一半的细胞迁移到残余的视杯中，形成第二个错位的神经节。睫状细胞和脉络膜细胞存在于神经节中，并且这些细胞经历上述事件的典型序列。图像
1. The development of transmission was studied in chick ciliary ganglia that had been deprived of their periphery during early embryonic development. 2. Peripherally deprived neurones in the ganglion differentiate in normal numbers and send functional axons into the post-ganglionic nerve. 3. Ganglion cells lacking a periphery follow the normal developmental sequence sending out transient dendrites at the time ganglion cell synapses are formed, and later retracting them when calyces appear. 4. Synapses, which appear functionally and ultrastructurally normal, form on all ganglion cells at the normal time and transmission is normal until Stage 34. Therefore information from the periphery is not required for ganglion cell synapse formation per se. 5. From Stages 35 to 38 most cells die, so that only 8% of the original number of cells remain in the operated ganglion. Transmission fails in many cells during this same time, but precedes cell loss by only a short time, so that deafferentation probably does not contribute substantially to cell death. 6. Both ciliary and choroid cells achieve full cytologic differentiation and are distinct from each other, indicating that the periphery is not required for the elaboration of the distinctive characteristics of these cells. Presynaptic fibres also differentiate into typical bouton as well as calyciform endings. Therefore, the type of preganglionic ending does not depend on ganglion cells establishing proper peripheral contacts. 7. It has not been possible to ascertain whether ganglion cell specificity is affected by the periphery. 8. Peripheral removal affects ganglion cell migration, so that two ganglia are formed. Approximately half of the cells migrate into the remnant optic cup forming a second misplaced ganglion. Ciliary and choroid cells occur in both ganglia and these cells go through the typical sequence of events described above. Images