Mitotic Spindle Assembly in Land Plants: Molecules and Mechanisms.
Mitotic Spindle Assembly in Land Plants: Molecules and Mechanisms.
复制标题
DOI：
10.3390/biology6010006
复制
发表时间：
2017-01-25
期刊：
影响因子：
4.2
通讯作者：
Goshima G
中科院分区：
文献类型：
--
作者：
Yamada M;Goshima G
在教科书中，植物的有丝分裂纺锤体常常与动物的纺锤体分开描述。它们在分子和机械水平上有什么不同？在本章中，我们首先概述了动物和陆生植物有丝分裂纺锤体组装的过程。我们接下来讨论基于数据库搜索的主轴装配系数的守恒。对超过100个动物纺锤体组装因子的研究表明，参与这一过程的基因在植物中非常保守，除了两个主要的缺失元件：中心体组分和细胞质动力蛋白复合物的亚基/调节子。然后，我们描述了纺锤体和成膜体组装机制的基础上获得的数据，从强大的基因功能丧失的分析，使用RNA干扰（RNAi）或突变体植物。最后，对植物纺锤体的研究前景进行了展望。
In textbooks, the mitotic spindles of plants are often described separately from those of animals. How do they differ at the molecular and mechanistic levels? In this chapter, we first outline the process of mitotic spindle assembly in animals and land plants. We next discuss the conservation of spindle assembly factors based on database searches. Searches of >100 animal spindle assembly factors showed that the genes involved in this process are well conserved in plants, with the exception of two major missing elements: centrosomal components and subunits/regulators of the cytoplasmic dynein complex. We then describe the spindle and phragmoplast assembly mechanisms based on the data obtained from robust gene loss-of-function analyses using RNA interference (RNAi) or mutant plants. Finally, we discuss future research prospects of plant spindles.