Identification of a membrane proteomic signature for human embryonic stem cells independent of culture conditions
Identification of a membrane proteomic signature for human embryonic stem cells independent of culture conditions
复制标题
DOI：
10.1016/j.scr.2008.06.001
复制
发表时间：
2008-09-01
影响因子：
1.2
通讯作者：
Kassem, Moustapha
中科院分区：
文献类型：
--
作者：
Harkness, Linda;Christiansen, Helle;Kassem, Moustapha
人类胚胎干细胞（hESC）的蛋白质组学分析可以确定细胞命运决定和自我更新的生物标志物。采用傅立叶变换LC-ESI-MS/MS和MS 3质谱，我们得到了一个膜蛋白质组签名之间的重叠培养在小鼠胚胎成纤维细胞（MEF）饲养和那些生长在MEF的无培养条件下的hESC。我们确定了444个跨膜或膜相关蛋白，其中157个是常见的两种培养条件。功能注释显示CD抗原（10%）、粘附蛋白（4%）、增殖相关蛋白（4%）、受体（41%）、转运蛋白（21%）、结构蛋白（5%）和具有各种功能的蛋白（15%）。此外，15种CD抗原和许多以前在hESC中未在蛋白质组水平观察到的表面标记分子，例如，确定了Nodal modulator 1、CD 222、transgelin-2和CD 81。总之，我们描述的第一个膜蛋白质组的人胚胎干细胞是独立的培养条件。这些数据可用于定义hESC的表型。(c)2008 Elsevier B. V.保留所有权利。
Proteomic profiling of human embryonic stem cells (hESC) can identify cell fate determination and self-renewal biomarkers. Employing Fourier transform LC-ESI-MS/MS and MS3 mass spectrometry, we obtained a membrane proteomic signature overlapping between hESC cultured on mouse embryonic fibroblast (MEF) feeders and those grown under MEF-free culture conditions. We identified 444 transmembrane or membrane-associated proteins, of which 157 were common between both culture conditions. Functional annotation revealed CD antigens (10%), adhesion proteins (4%), proliferation-associated proteins (4%), receptors (41%), transport proteins (21%), structural proteins (5%), and proteins with miscellaneous functions (15%). In addition, 15 CD antigens and a number of surface marker molecules not previously observed in hESC at a proteome level, e.g., Nodal modulator 1, CD222, transgelin-2, and CD81, were identified. In conclusion, we describe the first membrane proteome profile of hESC that is independent of culture conditions. These data can be used to define the phenotype of hESC. (c) 2008 Elsevier B.V. All rights reserved.