The lytB gene of Escherichia coli is essential and specifies a product needed for isoprenoid biosynthesis
The lytB gene of Escherichia coli is essential and specifies a product needed for isoprenoid biosynthesis
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DOI：
10.1128/jb.183.24.7403-7407.2001
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发表时间：
2001-12-01
影响因子：
3.2
通讯作者：
Masters, M
中科院分区：
文献类型：
--
作者：
McAteer, S;Coulson, A;Masters, M
LytB和GcpE由于与其他途径酶共分布，已被预测催化非甲戊酸途径中未知步骤的异戊二烯生物合成。我们构建了一个有条件的大肠杆菌lytB突变体，发现LytB是生存所必需的，LytB的耗尽会导致细胞裂解，这与该蛋白在类异戊二烯生物合成中的作用是一致的。酒精可以在假设的LytB步骤(S)之外转化为途径中间体，支持E.ColilytB突变体的有限生长。蛋白质结构的信息学分析表明，GcpE是Tim Barrel类的球状蛋白，LytB也是球状蛋白。提出了LytB和GcpE可能的生化作用。
LytB and GcpE, because they are codistributed with other pathway enzymes, have been predicted to catalyze unknown steps in the nonmevalonate pathway for isoprenoid biosynthesis. We constructed a conditional Escherichia coli lytB mutant and found that LytB is essential for survival and that depletion of LytB results in cell lysis, which is consistent with a role for this protein in isoprenoid biosynthesis. Alcohols which can be converted to pathway intermediates beyond the hypothesized LytB step(s) support limited growth of E. coli lytB mutants. An informatic analysis of protein structure suggested that GcpE is a globular protein of the TIM barrel class and that LytB is also a globular protein. Possible biochemical roles for LytB and GcpE are suggested.