Side-effects of resveratrol in HepG2 cells: Reduced pten and increased bcl-xl mRNA expression
Side-effects of resveratrol in HepG2 cells: Reduced pten and increased bcl-xl mRNA expression
复制标题
白藜芦醇在 HepG2 细胞中的副作用：减少 pten 和增加 bcl-xl mRNA 表达。
DOI：
10.3892/mmr.2012.1077
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发表时间：
2012-12-01
影响因子：
3.4
通讯作者：
Xie, Linna
中科院分区：
文献类型：
--
作者：
Zheng, Min;Chen, Ruifu;Xie, Linna
白藜芦醇是一种天然化合物，存在于红葡萄和红葡萄酒中，经常在人类饮食中食用。近年来，白藜芦醇在肝癌（HCC）中的化学预防和化学治疗潜力受到广泛关注。然而，白藜芦醇刺激的副作用是令人担忧的，因为对人类施用的剂量很高。本研究采用半定量和定量PCR方法研究白藜芦醇对HepG 2细胞pten和bcl-xl mRNA表达的影响。本研究表明，200 µmol/l白藜芦醇刺激12 h可抑制HepG 2增殖，降低pten，增加bcl-xl mRNA表达。这些数据表明，（a）白藜芦醇的抗癌机制不涉及诱导pten和抑制bcl-xl表达和（B）白藜芦醇诱导细胞自我保护反应，这是HepG 2细胞对白藜芦醇的细胞化学抗性的基础。
Resveratrol is a natural compound present in red grapes and red wine, and is often consumed in the human diet. The chemopreventive and chemotherapeutic potential of resveratrol in hepatocellular carcinoma (HCC), one of the most common types of liver cancer, has received much attention in recent years. However, the side-effects of resveratrol stimulation are of concern due to the high doses administered to humans. This study investigated the effects of resveratrol on the mRNA expression of pten and bcl-xl in HepG2 cells using semi-quantitative and quantitative PCR. This study demonstrated that 200 µmol/l resveratrol stimulation for 12 h resulted in the inhibition of HepG2 proliferation, reduced pten and increased bcl-xl mRNA expression. The data suggest that (a) the anticancer mechanism of resveratrol does not involve the induction of pten and inhibition of bcl-xl expression and (b) resveratrol induces a cellular self-protection response, which underlies the cellular chemoresistance against resveratrol in HepG2 cells.