Virus neutralizing antibody response in mice and dogs with a bicistronic DNA vaccine encoding rabies virus glycoprotein and canine parvovirus VP2.
Virus neutralizing antibody response in mice and dogs with a bicistronic DNA vaccine encoding rabies virus glycoprotein and canine parvovirus VP2.
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DOI：
10.1016/j.vaccine.2007.02.051
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发表时间：
2007-05
期刊：
影响因子：
5.5
通讯作者：
S. Patial;V. Chaturvedi;A. Rai;M. Saini;R. Chandra;Y. Saini;P. Gupta
中科院分区：
文献类型：
--
作者：
S. Patial;V. Chaturvedi;A. Rai;M. Saini;R. Chandra;Y. Saini;P. Gupta
通过将狂犬病糖蛋白和犬细小病毒（CPV） VP2基因亚克隆为双链载体，制备了犬狂犬病和细小病毒双链DNA疫苗。在体外鉴定了这两种蛋白的表达后，将双顺反子DNA疫苗注射到小鼠体内，并与单顺反子DNA疫苗诱导的免疫应答进行比较。双顺反子和单顺反子DNA疫苗免疫小鼠抗狂犬病和CPV的ELISA和病毒中和（VN）抗体反应无显著差异。此外，接种双反子或单反子狂犬DNA疫苗的小鼠对狂犬病毒的攻击具有显著相似的保护作用。同样，接种单顺反电子和双顺反电子DNA疫苗的狗产生了类似的抗狂犬病和CPV的VN抗体。本研究表明，双链DNA疫苗可诱导犬对狂犬病和CPV的病毒中和免疫反应。
A bicistronic DNA vaccine against rabies and parvovirus infection of dogs was developed by subcloning rabies glycoprotein and canine parvovirus (CPV) VP2 genes into a bicistronic vector. After characterizing the expression of both the proteins in vitro, the bicistronic DNA vaccine was injected in mice and induced immune response was compared with monocistronic DNA vaccines. There was no significant difference in ELISA and virus neutralizing (VN) antibody responses against rabies and CPV in mice immunized with either bicistronic or monocistronic DNA vaccine. Further, there was significantly similar protection in mice immunized with either bicistronic or monocistronic rabies DNA vaccine on rabies virus challenge. Similarly, dogs immunized with monocistronic and bicistronic DNA vaccines developed comparable VN antibodies against rabies and CPV. This study indicated that bicistronic DNA vaccine can be used in dogs to induce virus neutralizing immune responses against both rabies and CPV.