Quantum dot-labeled aptamer nanoprobes specifically targeting glioma cells
Quantum dot-labeled aptamer nanoprobes specifically targeting glioma cells
复制标题
专门针对神经胶质瘤细胞的量子点标记适体纳米探针
DOI：
10.1088/0957-4484/19/23/235105
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发表时间：
2008-06-11
期刊：
影响因子：
3.5
通讯作者：
Xie, Hai-Yan
中科院分区：
文献类型：
--
作者：
Chen, Xue-Chai;Deng, Yu-Lin;Xie, Hai-Yan
两种新技术，基于适体的特异性识别和基于量子点(QD)的荧光标记，在生物传感中变得越来越重要。在本研究中，将这两种技术结合在一起，通过将GBI-10适配子连接到量子点表面，构建了一种新型的荧光量子点标记适体(QD-APT)纳米探针。GBI-10是一种针对Tenascin-C的单链DNA(SsDNA)适配子，它作为一种主要的细胞外基质蛋白分布于胶质瘤细胞表面。QD-APT纳米探针可以识别人脑胶质瘤细胞表面的Tenascin-C，这将有助于开发新的简便、灵敏的胶质瘤体外诊断方法。QD-APT纳米探针具有荧光强、稳定性好、单分散性和均匀性等特点。此外，该探针制备方法具有通用性，有望为高通量、快速的生物传感检测和生物成像提供一种稳定的新型纳米探针。因此，可以开发用于实时和动态跟踪和成像的新方法。
Two new techniques, aptamer-based specific recognition and quantum dot (QD)-based fluorescence labeling, are becoming increasingly important in biosensing. In this study, these two techniques have been coupled together to construct a new kind of fluorescent QD-labeled aptamer (QD-Apt) nanoprobe by conjugating GBI-10 aptamer to the QD surface. GBI-10 is a single-stranded DNA (ssDNA) aptamer for tenascin-C, which distributes on the surface of glioma cells as a dominant extracellular matrix protein. The QD-Apt nanoprobe can recognize the tenascin-C on the human glioma cell surface, which will be helpful for the development of new convenient and sensitive in vitro diagnostic assays for glioma. The QD-Apt nanoprobe has particular features such as strong fluorescence, stability, monodispersity and uniformity. In addition, this probe preparation method is universal, so it is expected to provide a new type of stable nanoprobe for high-throughput and fast biosensing detection and bioimaging. New methods for real-time and dynamic tracking and imaging can be accordingly developed.