Mapping of RNA initiation sites by high doses of uv irradiation: evidence for three independent promoters within the left 11% of the Ad-2 genome.
Mapping of RNA initiation sites by high doses of uv irradiation: evidence for three independent promoters within the left 11% of the Ad-2 genome.
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映射%20of%20RNA%20起始%20位点%20by%20high%20doses%20of%20uv%20照射:%20证据%20for%20三%20独立%20启动子%20内%20the%20left%2011%%20of%20the%20Ad-2%
DOI：
10.1016/0042-6822(79)90447-1
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发表时间：
1979
期刊：
影响因子：
3.7
通讯作者：
J. Darnell
中科院分区：
文献类型：
--
作者：
M. Wilson;N. Fraser;J. Darnell
用Ad-2病毒感染的细胞被照射，使得在基因组中每500-1000个核苷酸引入UV诱导的损伤，从而导致RNA转录的过早终止。当用[3 H]尿苷标记时，这些细胞积累标记的启动子近端RNA。杂交后的大小分级的限制性片段的Ad-2基因组的RNA允许识别含有活性RNA起始位点的DNA序列。在感染周期的早期，在Ad-2基因组左侧11%的0 - 3.0和4.4 - 8.0限制性片段内发现了两个活性RNA起始位点。在晚期感染（15小时）期间，检测到额外的抗紫外线转录物，表明新激活的RNA起始位点（推测为蛋白IX）位于片段8.0 -11内。2.
Cells infected with Ad-2 virus were irradiated so that uv-induced lesions were introduced every 500–1000 nucleotides in the genome, consequently leading to the premature termination of RNA transcription. Such cells when labeled with [3H]uridine accumulate labeled promoter proximal RNA. Hybridization of this RNA after size fractionation to restriction fragments of the Ad-2 genome allowed the identification of DNA sequences containing active RNA initiation sites. Early during the infectious cycle two active RNA initiation sites were found within the left 11% of the Ad-2 genome within the0–3.0and4.4–8.0restriction fragments. During late infection (15 hr) an additional uv resistant transcript was detected indicating that a newly activated RNA initiation site, presumably for protein IX, resides within the fragment8.0–11.,2.