Hydrogen production by microorganisms in the hindgut of the termite <i>Reticulitermes speratus</i> under anaerobic and aerobic conditions
Hydrogen production by microorganisms in the hindgut of the termite <i>Reticulitermes speratus</i> under anaerobic and aerobic conditions
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白蚁<i>Reticulitermes speratus</i>后肠微生物在厌氧和需氧条件下产氢
DOI：
10.11257/jjeez.31.51
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Shuji Itakura
中科院分区：
文献类型：
--
作者：
Tomoyuki Konishi;Daichi Yamamoto;Kiwamu Umezawa;Shuji Itakura
精白蚁工种后肠匀浆在厌氧条件下产氢量(1.9Mol/白蚁)显著高于好氧条件下(0.3Mol/白蚁)96h的产氢量(27±1μ)。厌氧条件下的最大产氢速率(39nmol/白蚁/h)也显著高于好氧条件下的最大产氢速率(14nmol/白蚁/h)。在后肠匀浆中添加单糖可以增加厌氧和好氧条件下的产氢量。在厌氧条件下，添加阿拉伯糖(2 91μ/白蚁)、半乳糖(2 6 6μ/白蚁)和木糖(2 2 1μ摩尔/白蚁)的产氢量大于添加葡萄糖(70μ摩尔/白蚁)或甘露糖(94μ摩尔/白蚁)培养96h的产氢量；在好氧条件下，添加阿拉伯糖(2 6 7℃摩尔/白蚁)、半乳糖(2 2 9μ摩尔/白蚁)或木糖(195μ摩尔/白蚁)的产氢量高于添加葡萄糖(50μ摩尔/白蚁)或甘露糖(81μ摩尔/白蚁)的产氢量。
Hindgut homogenate from worker caste termites of Reticulitermes speratus generated a significantly greater amount of hydrogen under anaerobic conditions (1.9 μmol/termite) than that under aerobic conditions (0.3 μmol/termite) for 96 h at 27±1℃. The maximum hydrogen production rate was also significantly higher under anaerobic conditions (39 nmol/termite/h) than that under aerobic conditions (14 nmol/termite/h). The addition of monosaccharides to the hindgut homogenate increased the amounts of hydrogen produced under anaerobic and aerobic conditions. The amounts of hydrogen produced by adding arabinose (291 μmol/termite), galactose (266 μmol/termite), or xylose (221 μmol/termite) were greater than those obtained by adding glucose (70 μmol/termite) or mannose (94 μmol/termite) for an incubation period of 96 h at 27±1℃ under anaerobic conditions, while adding arabinose (267 μmol/termite), galactose (229 μmol/termite), or xylose (195 μmol/termite) resulted in greater hydrogen production than adding glucose (50 μmol/termite) or mannose (81 μmol/termite) under aerobic conditions.