Efficient extraction of polysaccharides from Lycium barbarum L. by aqueous two-phase system combined with tissue-smashing extraction
Efficient extraction of polysaccharides from Lycium barbarum L. by aqueous two-phase system combined with tissue-smashing extraction
复制标题
水两相体系结合组织粉碎提取高效提取枸杞多糖
DOI：
10.1016/j.indcrop.2022.115036
复制
发表时间：
2022-05-09
影响因子：
5.9
通讯作者：
Pei, Dong
中科院分区：
文献类型：
--
作者：
Hu, Jinxia;Liu, Jianfei;Pei, Dong
建立了一种基于组织破碎萃取辅助双水相体系（TSE-ATPS）提取枸杞子的有效方法。水果多糖（LBPs）。考察了由3种不同盐类组成的乙醇/盐双水相体系（ATPS），从中筛选出17.86%（NH 4）2SO 4（w/ w）和28.86%乙醇（w/w）的体系。结果表明，在料液比1：30（m/v）、粉碎功率8000 rpm（8 krpm）、提取时间4 min的条件下，枸杞多糖的提取率为24.79 mg/ g。与热水提取法和超声辅助提取法相比，TSE-ATPS（（NH 4）2SO 4/乙醇）提高了枸杞多糖的提取率。用不同方法提取的LBP具有不同的化学结构和单糖组成。当ATPS由K2 HPO 4/乙醇和NaH 2 PO 4/乙醇组成时，表现出上级的抗氧化活性。因此，TSE-ATPS作为一种创新的、有前途的、快速破碎提取技术，为天然产物中多糖的提取和分离提供了更好的选择。
An effective method based on tissue-smashing extraction assisted aqueous two-phase system (TSE-ATPS) was developed for extracting Lycium barbarum L. fruits polysaccharides (LBPs). The ethanol/salt aqueous two-phase system (ATPS) composed of three different salts was evaluated, from which the system of 17.86% (NH4)2SO4 (w/ w) and 28.86% ethanol (w/w) was chosen. The conditions were as follows: solid-to-solvent ratio 1:30 (m/v), tissue-smashing power 8000 rpm (8 krpm), extraction time 4 min and the extraction yield of LBPs was 24.79 mg/ g. Compared with hot water extraction (HWE) and ultrasound-assisted extraction (UAE), TSE-ATPS ((NH4)2SO4/ ethanol) improved the extraction yield of LBPs. LBPs extracted using various methods have different chemical structures and monosaccharide compositions. Superior antioxidant activity was demonstrated when the ATPS was composed of K2HPO4/ethanol and NaH2PO4/ethanol. Therefore, as an innovative, promising, and rapid crushing extraction technology, TSE-ATPS provides a better choice for extraction and separation of polysaccharides from natural products.