Characterization of the steady-state population of messenger RNA and its poly(adenylic acid) segment in mammalian cells.
Characterization of the steady-state population of messenger RNA and its poly(adenylic acid) segment in mammalian cells.
复制标题
哺乳动物细胞中信使 RNA 及其聚腺苷酸片段稳态群体的表征。
DOI：
10.1021/bi00719a026
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发表时间：
1974
期刊：
影响因子：
2.9
通讯作者：
G. Brawerman
中科院分区：
文献类型：
--
作者：
W. Jeffery;G. Brawerman
William R. Jeffery* 和乔治Brawerman* 摘要：小鼠肉瘤和兔网织红细胞多聚核糖体中含有多聚腺苷酸的RNA，采用基于与放射性多聚腺苷酸形成复合物的方法进行表征。EDTA处理网织红细胞多聚核糖体释放出含有聚（A）的物质作为均匀的颗粒，而同样的处理应用于肉瘤多聚核糖体释放出高度异质的颗粒群。从稳定状态的肉瘤多聚核糖体RNA中获得的poly（A）片段比从新合成的RNA中获得的poly（A）片段显示出更不均匀的沉积特征。其平均沉降系数也较低。基于观察到的沉降系数，肉瘤多聚体RNA的稳态poly（A）片段的平均大小的估计为130个残基，而肉瘤多聚体RNA的稳态poly（A）片段的平均大小的估计为130个残基。
William R. Jeffery* and George Brawerman* abstract: The poly (A) containing RNA of mouse sarcoma and rabbit reticulocyte polysomes was characterized using a method based on complex formationwith radioactive poly (U). EDTA treatment of reticulocyte polysomes releases the poly (A) containing material as a homogeneous particle, while the same treatment applied to sarcoma poly-somes releases a highly heterogeneous population of parti-cles. The poly (A) segments obtained from steady-state sar-coma polysomal RNA exhibit a more heterogeneous sedi-mentation profile than the poly (A) from newly synthesized RNA. Their average sedimentationcoefficient is also lower. The estimate of the average size of the steady-state poly (A) segment of sarcoma polysomal RNA, based on the observed sedimentation coefficient, is 130 residues, compared to the