Micro-Raman spectroscopy detects individual neoplastic and normal hematopoietic cells
Micro-Raman spectroscopy detects individual neoplastic and normal hematopoietic cells
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DOI：
10.1529/biophysj.105.066761
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发表时间：
2006-01-01
影响因子：
3.4
通讯作者：
Huser, T
中科院分区：
文献类型：
--
作者：
Chan, JW;Taylor, DS;Huser, T
目前用于鉴定肿瘤细胞并将其与其正常对应物区分开的方法通常是非特异性的、缓慢的、生物干扰的或其组合。在这里，我们表明，单细胞显微拉曼光谱避免了这些缺点，并可用于区分未固定的正常人淋巴细胞和转化的Jurkat和Raji淋巴细胞系的基础上，他们的生物分子拉曼签名。我们证明，单细胞拉曼光谱提供了一个高度可重复的每种细胞类型的生物分子指纹。特征峰，主要是由于不同的DNA和蛋白质浓度，允许区分正常淋巴细胞与转化淋巴细胞具有高置信度（p
Current methods for identifying neoplastic cells and discerning them from their normal counterparts are often nonspecific, slow, biologically perturbing, or a combination thereof. Here, we show that single-cell micro-Raman spectroscopy averts these shortcomings and can be used to discriminate between unfixed normal human lymphocytes and transformed Jurkat and Raji lymphocyte cell lines based on their biomolecular Raman signatures. We demonstrate that single-cell Raman spectra provide a highly reproducible biomolecular fingerprint of each cell type. Characteristic peaks, mostly due to different DNA and protein concentrations, allow for discerning normal lymphocytes from transformed lymphocytes with high confidence (p