Proteomics-Based Comparative Mapping of the Secretomes of Human Brown and White Adipocytes Reveals EPDR1 as a Novel Batokine
Proteomics-Based Comparative Mapping of the Secretomes of Human Brown and White Adipocytes Reveals EPDR1 as a Novel Batokine
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DOI：
10.1016/j.cmet.2019.10.001
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发表时间：
2019-11-05
期刊：
影响因子：
29
通讯作者：
Scheele, Camilla
中科院分区：
文献类型：
--
作者：
Deshmukh, Atul S.;Peijs, Lone;Scheele, Camilla
白色脂肪组织分泌的脂肪因子在代谢串扰和体内平衡中发挥作用，而棕色脂肪分泌组则较少被探索。我们对成人锁骨上棕色脂肪和皮下白色脂肪的脂肪细胞培养基进行了高灵敏度的蛋白质组学研究。我们确定了471种潜在的分泌蛋白，涵盖了有趣的类别，如激素、生长因子、细胞外基质蛋白和补体系统蛋白，它们在棕色和白色脂肪细胞之间受到不同的调节。在棕色脂肪细胞中，共有101种蛋白被定量，其中包括室管素相关蛋白1 （EPDR1）。在人血浆中检测到EPDR1，功能研究表明EPDR1在脂肪形成过程中的产热决定中起作用。总之，我们报告了棕色和白色人类脂肪细胞分泌组之间的实质性差异，并确定了可能是人类代谢重要调节因子的新的候选细胞因子。
Adipokines secreted from white adipose tissue play a role in metabolic crosstalk and homeostasis, whereas the brown adipose secretome is less explored. We performed high-sensitivity mass-spectrometry-based proteomics on the cell media of human adipocytes derived from the supraclavicular brown adipose and from the subcutaneous white adipose depots of adult humans. We identified 471 potentially secreted proteins covering interesting categories such as hormones, growth factors, extracellular matrix proteins, and proteins of the complement system, which were differentially regulated between brown and white adipocytes. A total of 101 proteins were exclusively quantified in brown adipocytes, and among these was ependymin-related protein 1 (EPDR1). EPDR1 was detected in human plasma, and functional studies suggested a role for EPDR1 in thermogenic determination during adipogenesis. In conclusion, we report substantial differences between the secretomes of brown and white human adipocytes and identify novel candidate batokines that can be important regulators of human metabolism.