Cloning and sequence analysis of cDNA species coding for the two subunits of inhibin from bovine follicular fluid.
Cloning and sequence analysis of cDNA species coding for the two subunits of inhibin from bovine follicular fluid.
复制标题
编码牛卵泡液抑制素两个亚基的 cDNA 物种的克隆和序列分析。
DOI：
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发表时间：
1986
影响因子：
11.1
通讯作者：
J. Findlay
中科院分区：
文献类型：
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作者：
R. Forage;J. Ring;R. Brown;B. McInerney;G. Cobon;R. P. Gregson;David Robertson;F. Morgan;M. Hearn;J. Findlay
通过克隆和分析来自牛颗粒细胞mRNA的cDNA种类，已经阐明了58-kDa形式的激素类胡萝卜素的43-kDa（A）和15-kDa（B）亚基的一级氨基酸结构。A亚基（Mr = 32，298）是具有两个潜在的N-糖基化位点和两个潜在的蛋白水解加工位点的300个氨基酸的蛋白质，并且具有60个氨基酸的前原区。成熟的B亚基（Mr = 12，977）是由单独的mRNA合成的116个氨基酸的蛋白质。这些数据证实，也从牛卵泡液中分离出的31-kDa形式的具有20 kDa（Ac）和15 kDa（B）亚基的Bclarbin是通过A亚基的蛋白水解加工从58-kDa形式衍生而来的。
The primary amino acid structures of the 43-kDa (A) and 15-kDa (B) subunits of the 58-kDa form of the hormone inhibin have been elucidated by cloning and analysis of cDNA species derived from bovine granulosa cell mRNA. The A subunit (Mr = 32,298) is a protein of 300 amino acids with two potential N-glycosylation sites and two potential proteolytic processing sites and has a pre-pro region of 60 amino acids. The mature B subunit (Mr = 12,977) is a protein of 116 amino acids synthesized from a separate mRNA. These data establish that a 31-kDa form of inhibin also isolated from bovine follicular fluid, with subunits of 20 kDa (Ac) and 15 kDa (B), is derived from the 58-kDa form by proteolytic processing of the A subunit.