Identification of the transcriptional promoters in the proximal regions of human microRNA genes
Identification of the transcriptional promoters in the proximal regions of human microRNA genes
复制标题
人类 microRNA 基因近端区域转录启动子的鉴定
DOI：
10.1007/s11033-010-0535-y
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发表时间：
2011-08-01
影响因子：
2.8
通讯作者：
Liao, Wei-Ping
中科院分区：
文献类型：
--
作者：
Long, Yue-Sheng;Deng, Guang-Fei;Liao, Wei-Ping
为了鉴定人类microRNA（miRNA）基因近端区域的转录启动子，我们分析了基因间miRNAs和内含子miRNAs的5'侧翼区。通过TSSG程序预测，发现内含子-s miRNA基因中至少有一个启动子的比例显著低于基因间miRNA基因和内含子-a miRNA基因。超过一半的miRNA基因只有一个启动子，不到20%的miRNA基因在5 kb上游区域有三个以上的启动子。所有潜在的启动子随机分布在这些区域内。大约60%的miRNA启动子具有TATA样盒，显著高于所有人类启动子。荧光素酶报告基因分析显示，30个启动子中的22个驱动HEK-293细胞中的基因表达，表明启动子预测的高准确性。本研究为进一步研究人类miRNA基因的转录调控机制奠定了基础。
To identify the transcriptional promoters in the proximal regions of human microRNA (miRNA) genes, we analyzed the 5' flanking regions of intergenic miRNAs and intronic miRNAs. With the TSSG program prediction, we found that the ratio of intronic-s miRNA genes with a least one promoter was significantly lower than those of intergenic miRNA genes and intronic-a miRNA genes. More than half of the miRNA genes have only one promoter and less than 20% of the miRNA genes have more than three promoters in the 5-kb upstream regions. All potential promoters are randomly distributed within these regions. Approximately 60% of the miRNA promoters have a TATA-like box, being significantly higher than that of all human promoters. Luciferase reporter assays showed that 22 of the 30 promoters drove gene expression in HEK-293 cells, indicating a high accuracy of the promoter prediction. This study lays a foundation for future investigation into the transcriptional regulatory mechanisms of human miRNA genes.