The CBP co-activator is a histone acetyltransferase
The CBP co-activator is a histone acetyltransferase
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DOI：
10.1038/384641a0
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发表时间：
1996-12-19
期刊：
影响因子：
64.8
通讯作者：
Kouzarides, T
中科院分区：
文献类型：
--
作者：
Bannister, AJ;Kouzarides, T
CBP蛋白通过直接接触DNA结合激活剂（1-7）来充当许多不同转录因子的转录适配器。 CBP被认为刺激转录的一种机制是通过向启动子募集组蛋白乙酰转移酶（HAT）P/CAF（8）。在这里，我们表明CBP具有内在的帽子活动。 CBP的HAT结构域与转录激活剂E1A的结合位点相邻。尽管E1A从CBP中取代P/CAF，但不会破坏与CBP相关的HAT活性。因此，E1A与CBP复合时会带有HAT活动。因此，将CBP相关的HAT活性靶向特定启动子可能是E1A充当转录激活剂的机制。
THE CBP protein acts as a transcriptional adaptor for many different transcription factors by directly contacting DNA-bound activators(1-7). One mechanism by which CBP is thought to stimulate transcription is by recruiting the histone acetyltransferase (HAT) P/CAF to the promoters(8). Here we show that CBP has intrinsic HAT activity. The HAT domain of CBP is adjacent to the binding site for the transcriptional activator E1A. Although E1A displaces P/CAF from CBP, it does not disrupt the CBP-associated HAT activity. Thus E1A carries HAT activity when complexed with CBP. Targeting CBP-associated HAT activity to specific promoters may therefore be a mechanism by which E1A acts as a transcriptional activator.