Analysis of the uterine lumen in fertility-classified heifers: II. Proteins and metabolites
Analysis of the uterine lumen in fertility-classified heifers: II. Proteins and metabolites
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DOI：
10.1093/biolre/ioz197
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发表时间：
2020-03-01
影响因子：
3.6
通讯作者：
Spencer, Thomas E.
中科院分区：
文献类型：
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作者：
Moraes, Joao G. N.;Behura, Susanta K.;Spencer, Thomas E.
牛孕体的存活和生长依赖于子宫内膜分泌物或组织营养菌。以前，连续囊胚移植用于将小母牛分为高生育力（HF）、低生育力（SF）或不育（IF）。在这里，我们调查了特定的组织营养成分（蛋白质和代谢产物）在子宫腔的第17天生育分类的小母牛。干扰素tau（IFNT）在妊娠HF的子宫腔液（ULF）中比SF动物更丰富，因为HF小母牛的孕体更长。然而，没有观察到子宫内膜表达的经典和非经典干扰素刺激基因（ISGs）的差异，表明IFNT信号在怀孕的HF和SF小母牛的子宫内膜是相似的。怀孕显着增加了ULF中多种蛋白质的丰度。基于功能注释，大量参与能量代谢，氧化应激，氨基酸代谢，细胞增殖和分化的蛋白质的丰度在ULF怀孕HF比SF小母牛。代谢组学分析发现，妊娠仅改变HF小母牛的ULF代谢组组成。与SF小母牛相比，妊娠HF小母牛ULF中增加的大多数代谢产物与能量和氨基酸代谢相关。ULF蛋白质组和代谢组中观察到的差异被假设为影响子宫容受性，其后果是对孕体发育和生育力分类的小母牛的存活。
Survival and growth of the bovine conceptus is dependent on endometrial secretions or histotroph. Previously, serial blastocyst transfer was used to classify heifers as high fertile (HF), subfertile (SF), or infertile (IF). Here, we investigated specific histotroph components (proteins and metabolites) in the uterine lumen of day 17 fertility-classified heifers. Interferon tau (IFNT) was more abundant in uterine lumenal fluid (ULF) of pregnant HF than SF animals as the conceptus was longer in HF heifers. However, no differences in endometrial expression of selected classical and nonclassical interferon-stimulated genes (ISGs) were observed, suggesting that IFNT signaling in the endometrium of pregnant HF and SF heifers was similar. Pregnancy significantly increased the abundance of several proteins in ULF. Based on functional annotation, the abundance of a number of proteins involved in energy metabolism, oxidative stress, amino acid metabolism, and cell proliferation and differentiation were greater in the ULF of pregnant HF than SF heifers. Metabolomics analysis found that pregnancy only changed the metabolome composition of ULF from HF heifers. The majority of the metabolites that increased in the ULF of pregnant HF as compared to SF heifers were associated with energy and amino acid metabolism. The observed differences in ULF proteome and metabolome are hypothesized to influence uterine receptivity with consequences on conceptus development and survival in fertility-classified heifers.Pregnancy induced changes in the uterine lumen are dysregulated in subfertile heifers.