No Evidence of rVSV-Ebola Virus Vaccine Replication or Dissemination in the Sand Fly Phlebotomus papatasi
No Evidence of rVSV-Ebola Virus Vaccine Replication or Dissemination in the Sand Fly Phlebotomus papatasi
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DOI：
10.4269/ajtmh.20-0951
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发表时间：
2021-03-01
影响因子：
3.3
通讯作者：
Pitt, M. Louise M.
中科院分区：
文献类型：
--
作者：
Haddow, Andrew D.;Rowland, Tobin E.;Pitt, M. Louise M.
在接种印第安纳州重组水泡性口炎病毒印第安纳血清型埃博拉病毒(rVSV-EBOV)减毒活疫苗后，人们可能会出现与疫苗相关的一过性病毒血症。为了研究东半球沙蝇在摄食病毒者后传播这种疫苗的可能性，我们给实验室饲养的帕帕塔西白头翁喂了含有7.2log10个rVSV-EBOV空斑形成单位的人工血粉。在喂食后7天(n=75)或15天(n=75)采集的任何帕帕塔西原虫的身体或腿部均未检测到复制或传播。这些结果表明，rVSV-EBOV在帕帕塔西原虫中复制和传播的潜力很低，该物种的地理分布范围从摩洛哥到西南亚，甚至北至南欧。
Following vaccination with the live attenuated, recombinant vesicular stomatitis virus Indiana serotype Ebola virus (rVSV-EBOV) vaccine, persons may exhibit a transient vaccine-associated viremia. To investigate the potential for Old World sand flies to transmit this vaccine following feeding on a viremic person, we fed laboratory-reared Phlebotomus papatasi an artificial blood meal containing 7.2 log10 plaque-forming units of rVSV-EBOV. Replication or dissemination was not detected in the body or legs of any P. papatasi collected at seven (n = 75) or 15 (n = 75) days post-feed. These results indicate a low potential for rVSV-EBOV to replicate and disseminate in P. papatasi, a species whose geographic distribution ranges from Morocco to southwest Asia and as far north as southern Europe.