Rhombic-Shaped Nanostructures and Mechanical Properties of 2D DNA Origami Constructed with Different Crossover/Nick Designs
Rhombic-Shaped Nanostructures and Mechanical Properties of 2D DNA Origami Constructed with Different Crossover/Nick Designs
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不同交叉/切口设计构建的 2D DNA 折纸的菱形纳米结构和机械性能
DOI：
10.1002/smll.201702028
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发表时间：
2017
期刊：
影响因子：
13.3
通讯作者：
Tabata Osamu
中科院分区：
文献类型：
--
作者：
Ma Zhipeng;Huang Yunfei;Park Seongsu;Kawai Kentaro;Kim Do-Nyun;Hirai Yoshikazu;Tsuchiya Toshiyuki;Yamada Hirofumi;Tabata Osamu
DNA折纸方法能够制造各种纳米结构和纳米器件，但它们的有效使用取决于对其结构和机械特性的理解以及基本结构特征的影响。采用调频原子力显微镜直接表征水溶液中基于不同交叉/刻痕设计的二维DNA折纸的交叉区域。在DNA折纸中，每3、4或6个DNA转一次交叉，在DNA螺旋之间的柔性交叉影响下形成菱形纳米结构。根据其他地方报道的螺旋间静电力，交叉设计的弯曲刚度仅为DNA螺旋的三分之一，并且3转交叉设计的菱形纳米结构的测量节距可以忽略弯曲。为了评估其结构完整性的稳健性，使用力控制原子力显微镜对它们进行了有意和同时的受力。DNA交叉被证实对结构稳健性有稳定作用，而刻痕则有相反的作用。本文所揭示的DNA折纸的结构和力学特性以及交叉和缺口的影响可以为设计具有特定和理想性能的多功能DNA折纸结构提供必要的信息。
DNA origami methods enable the fabrication of various nanostructures and nanodevices, but their effective use depends on an understanding of their structural and mechanical properties and the effects of basic structural features. Frequency‐modulation atomic force microscopy is introduced to directly characterize, in aqueous solution, the crossover regions of sets of 2D DNA origami based on different crossover/nick designs. Rhombic‐shaped nanostructures formed under the influence of flexible crossovers placed between DNA helices are observed in DNA origami incorporating crossovers every 3, 4, or 6 DNA turns. The bending rigidity of crossovers is determined to be only one‐third of that of the DNA helix, based on interhelical electrostatic forces reported elsewhere, and the measured pitches of the 3‐turn crossover design rhombic‐shaped nanostructures undergoing negligible bending. To evaluate the robustness of their structural integrity, they are intentionally and simultaneously stressed using force‐controlled atomic force microscopy. DNA crossovers are verified to have a stabilizing effect on the structural robustness, while the nicks have an opposite effect. The structural and mechanical properties of DNA origami and the effects of crossovers and nicks revealed in this paper can provide information essential for the design of versatile DNA origami structures that exhibit specified and desirable properties.