Remarkable conservation of structure among intermediate filament genes
Remarkable conservation of structure among intermediate filament genes
复制标题
中间丝基因结构显着保守
DOI：
10.1016/0092-8674(84)90456-2
复制
发表时间：
1984
期刊：
影响因子：
64.5
通讯作者：
E. Fuchs
中科院分区：
文献类型：
--
作者：
D. Marchuk;S. McCrohon;E. Fuchs
利用与50 kd人表皮角蛋白部分mRNA互补的克隆cDNA，我们筛选了一个人类基因组文库，并分离和测序了编码该角蛋白的基因。将角蛋白基因与非常遥远的vimentin基因进行比较，使我们能够探索中间丝（IF）亚基结构的进化守恒与IF基因结构守恒之间的关系。我们的研究结果表明，不仅IF蛋白的二级结构，而且其基因的结构骨架在整个进化过程中一直保持不变。尽管蛋白质和核酸序列具有相当大的灵活性，但这些特征仍然存在。令人惊讶的是，尽管这两个基因中的内含子位置高度保守，但它们似乎并不对应于所有IF亚基共有的结构域的边界。
Using a cloned cDNA complementary to a portion of the mRNA for the 50 kd human epidermal keratin, we have screened a human genomic library and have isolated and sequenced the gene encoding this keratin. A comparison of the keratin gene with the very distantly related vimentin gene has enabled us to explore the relation between the evolutionary conservation of structure in intermediate filament (IF) subunits and the conservation of structure in IF genes. Our results reveal that not only the secondary structure of the IF proteins, but also the structural skeleton of their genes, has been maintained throughout evolution. These characteristics have persisted despite considerable flexibility in both protein and nucleic acid sequence. Surprisingly, although the positions of the introns within these two genes are highly conserved, they do not seem to correspond to the boundaries of the structural domains common to all IF subunits.