Gene expression in skeletal muscle
Gene expression in skeletal muscle
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DOI：
10.1042/bst0300285
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发表时间：
2002-04-01
影响因子：
3.9
通讯作者：
Goldspink, G
中科院分区：
文献类型：
--
作者：
Goldspink, G
肌肉有一种内在的能力，可以根据活动改变其质量和表型。这一过程涉及基因表达的定量和质变，包括编码不同类型分子马达的肌球蛋白重链等基因的表达。这一点，以及代谢基因的差异表达，导致了疲劳抵抗和功率输出的改变。肌肉量的调节涉及自分泌和全身因素。我们从运动肌肉中克隆了胰岛素样生长因子- i （IGF-I）的局部和全身亚型的cdna。虽然不同的同种异构体是通过选择性剪接从igf - 1基因衍生出来的，但其中一种的RNA转录物只有在损伤和/或机械活动后才能检测到。因此，这种蛋白质被称为机械生长因子（MGF）。由于阅读框移位，与全身或肝脏IGF-I相比，MGF具有不同的3'序列和不同的作用模式。虽然MGF被称为生长因子，但它可能作为局部修复因子受到调节。
Muscle has an intrinsic ability to change its mass and phenotype in response to activity. This process involves quantitative and qualitative changes in gene expression, including that of the myosin heavy chain isogenes that encode different types of molecular motors. This, and the differential expression of metabolic genes, results in altered fatigue resistance and power output. The regulation of muscle mass involves autocrine as well as systemic factors. We have cloned the cDNAs of local and systemic isoforms of insulin-like growth factor-I (IGF-I) from exercised muscle. Although different isoforms are derived from the IGF-I gene by alternative splicing, the RNA transcript of one of them is only detectable following injury and/or mechanical activity. Thus this protein has been called mechano growth factor (MGF). Because of a reading-frame shift, MGF has a different 3' sequence and a different mode of action compared with systemic or liver IGF-I. Although MGF has been called a growth factor, it may be regulated as a local repair factor.