Requirement for the 3' flanking region of the bovine growth hormone gene for accurate polyadenylylation.
Requirement for the 3' flanking region of the bovine growth hormone gene for accurate polyadenylylation.
复制标题
牛生长激素基因的 3 侧翼区域需要准确的聚腺苷酸化。
DOI：
10.1073/pnas.81.13.3944
复制
发表时间：
1984
影响因子：
11.1
通讯作者：
Rottman,FM
中科院分区：
文献类型：
--
作者：
Woychik,RP;Lyons,RH;Post,L;Rottman,FM
我们检查了从3'延伸至牛生长激素基因的聚腺苷酸化位点的序列是否包含影响生长激素mRNA的聚腺苷酸化的任何信号。为此，使用该基因的克隆拷贝转染 COS-1 细胞，每个拷贝在野生型聚腺苷酸化位点 3' 处含有不同长度的生长激素特异性序列。使用 S1 核酸酶作图程序分析由外源添加的生长激素基因产生的 mRNA 上的聚腺苷酸化位点。我们发现，含有 84 个碱基对的 3' 侧翼序列的基因能够产生精确的聚腺苷酸化 mRNA。另一方面，仅包含1、10或13个碱基对的3'侧翼序列的基因主要在野生型位置上游或下游的离散位点处多聚腺苷酸化。使用计算机程序，我们检查了初级生长激素转录本上的二级结构是否与 mRNA 聚腺苷酸化位点相关。
We examined whether the sequence extending 3' to the polyadenylylation site of the bovine growth hormone gene contains any signal that affects the polyadenylylation of the growth hormone mRNA. For this purpose, cloned copies of this gene, each containing a different length of growth hormone-specific sequence 3' to the wild-type polyadenylylation site, were used to transfect COS-1 cells. The polyadenylylation site on the mRNAs produced from the exogenously added growth hormone genes were analyzed with an S1 nuclease mapping procedure. We found that a gene containing 84 base pairs of its own 3' flanking sequence is capable of producing an accurately polyadenylylated mRNA. On the other hand, genes containing only 1, 10, or 13 base pairs of 3' flanking sequence were principally polyadenylylated at discrete sites either upstream or downstream from the wild-type position. Using a computer program, we examined whether secondary structures on the primary growth hormone transcript correlated with the site where the mRNA is polyadenylylated.