Mitochondrial DNA CA dinucleotide repeats in Koreans: the presence of length heteroplasmy
Mitochondrial DNA CA dinucleotide repeats in Koreans: the presence of length heteroplasmy
复制标题
DOI：
10.1007/s00414-004-0487-7
复制
发表时间：
2005-01-01
影响因子：
2.1
通讯作者：
Shin, KJ
中科院分区：
文献类型：
--
作者：
Chung, UH;Lee, HY;Shin, KJ
线粒体DNA HV 3区含有CA二核苷酸重复序列，具有长度多态性。为了分析这一法医学目的，我们设计了一个荧光标记的PCR引物集的短扩增产物，并进行了基因分型，通过毛细管电泳。在500名无关的韩国人中，共观察到4个等位基因，重复单位为4 - 7，遗传多样性估计为0.5120。有趣的是，三个个体显示出两个或三个长度变异，即长度异质性。
The mitochondrial DNA HV3 region contains CA dinucleotide repeats which display length polymorphism. To analyze this for forensic purposes, we designed a fluorescence-labelled PCR primer set for short amplification products and carried out genotyping by using capillary electrophoresis. A total of 4 alleles with 4 - 7 repeat units were observed and the genetic diversity was estimated to be 0.5120 in 500 unrelated Koreans. Interestingly, three individuals showed two or three length variants, i.e. length heteroplasmy.