Involvement of cell fatty acid composition and lipid metabolism in adhesion mechanism of Listeria monocytogenes
Involvement of cell fatty acid composition and lipid metabolism in adhesion mechanism of Listeria monocytogenes
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DOI：
10.1016/j.ijfoodmicro.2007.11.039
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发表时间：
2008-03-31
影响因子：
5.4
通讯作者：
Guerzoni, Maria Elisabetta
中科院分区：
文献类型：
--
作者：
Gianotti, Andrea;Serrazarietti, Diana;Guerzoni, Maria Elisabetta
根据生物膜形成能力筛选出两株单核细胞增生李斯特菌，并对其粘附细胞和漂浮细胞的脂肪酸组成进行了研究。Anteiso-15：0、iso-C15：0和anfeiso-17：0是漂浮细胞中的主要FA。另一方面，两株菌的粘附细胞呈现以直链脂肪酸如十六烷酸（16：0）和十八烷酸（18：0）为主的脂肪酸谱。当粘附和自由漂浮的细胞的两个菌株分别暴露于酸胁迫，增加的个别和总的分支脂肪酸观察到特别是在两个菌株的漂浮细胞。在酸胁迫条件下，生物膜形成能力较低的菌株贴壁细胞内直链中链脂肪酸（FFA）大量积累，而生物膜形成能力较高的菌株CH 4在培养上清中释放出直链长链和中链游离脂肪酸（FFA）。后一种菌株暴露于其自身的上清液（条件培养基-CM）增强了培养基中C16：0和C18：0的积累。特别是在菌株的上清液中检测到不寻常数量的直链中链烃，如正癸烷和正十三烷。此外，长链烃十九烷被释放的两个菌株时，暴露于酸胁迫。由于粘附与具有玻璃和离子相互作用的细胞的疏水连接有关，因此这些疏水分子可以在粘附和去粘附机制中发挥关键作用。(C)2007 Elsevier B. V.保留所有权利。
The cell ratty acid (FA) composition of adhered and floating cells of two Listeria monocytogenes strains, selected on the basis of the biofilm forming ability, was taken into consideration. Anteiso-15:0, iso-C15:0 and anfeiso-17:0 resulted the principal FAs in floating cells. On the other hand the adhered cells of the two strains presented a FAs profile characterized by a prevalence of straight FAs Such as hexadecanoic acid (16:0) and octadecanoic acid (18:0). When adhered and free floating cells of the two strains were separately exposed to acid stress, an increase of the individual and total branched FAs was observed particularly in the floating cells of the two strains. The acid stress gave rise in the adhered cells of strain endowed with the lower biofilm forming ability to a relevant intracellular accumulation of straight medium chain FAs.Straight long chain and medium chain free fatty acids (FFAs) were released in the colture supernatants particularly by the strain CH4 endowed with a high biofilm forming ability. The exposure of the latter strain to its own supernatants (conditioned media-CMs) enhanced the C 16:0 and C18:0 accumulation in the medium. An unusual quantity of straight medium chain hydrocarbons like n-decane and n-tridecane has been detected particularly in the supernatants of strain. Moreover, long chain hydrocarbons up to nonadecane were released by both strains when exposed to acid stress. Since adhesion is connected with the hydrophobic link of the cells with glass and ionic interactions, these hydrophobic molecules Could exercise a key role in the adhesion and de-adhesion mechanisms. (C) 2007 Elsevier B.V. All rights reserved.