Oral supplementation with fish oil reduces dryness and pruritus in the acetone-induced dry skin rat model
Oral supplementation with fish oil reduces dryness and pruritus in the acetone-induced dry skin rat model
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DOI：
10.1016/j.jdermsci.2015.06.015
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发表时间：
2015-09-01
影响因子：
4.6
通讯作者：
Rodrigues, Luis M.
中科院分区：
文献类型：
--
作者：
Barcelos, Raquel C. S.;de Mello-Sampay, Cris-Tina;Rodrigues, Luis M.
背景：干燥症和特应性皮炎患者经常出现瘙痒和不适。一些研究表明饮食在皮肤病理生理中起着重要作用。目的：本研究通过一个与瘙痒相关的动物模型，评估了饮食脂肪酸对皮肤生理的影响，该模型在添加和不添加鱼油(鱼油是多不饱和脂肪酸（PUFA）的来源，尤其是omega -3 （n-3）。方法：雄性Wistar大鼠分为未添加（对照组）和添加鱼油（3 g/kg/d）灌胃90 d。每30天，在背部皮肤暴露于丙酮诱导瘙痒相关皮肤干燥之前和之后，评估刮伤和皮肤参数（经皮失水（TEWL）、水合作用和局部血流量）。在研究结束时，动物被处死，并收集皮肤样本进行GC-FID脂肪酸组成分析。结果：补充鱼油降低了TEWL，增加了皮肤水分，从第60天开始变化显著，而皮肤微循环没有变化。它还减轻了丙酮引起的皮肤屏障改变，表现为更快地溶解水分和水合作用，消除皮肤干燥引起的瘙痒相关抓伤。这些变化与鱼油补充导致的皮肤脂肪酸掺入模式（n-3含量丰富，n-6/n-3 < 5）的改变有关。结论：皮肤屏障动力学似乎受fon -3 PUFA的影响，对皮肤干燥引起的抓痕行为有抑制作用。因此，长期补充富含n-3 PUFA的营养素可能会加强和恢复皮肤的完整性和功能。(C) 2015日本皮肤病研究学会。爱思唯尔爱尔兰有限公司出版。版权所有。
Background: Pruritus and discomfort are often present in patients with xerosis and atopic dermatitis. Several studies suggest an important role of diet in skin pathophysiology.Objective: This study evaluated the effect of dietary fatty acids in the skin physiology via an itch-related animal model with and without supplementation with fish oil (FO), a source of polyunsaturated fatty acids (PUFA), especially omega 3 (n-3).Methods: Male Wistar rats were divided into two groups non-supplemented (control) and supplemented with FO (3 g/kg/day) by gavage for 90 days. Every 30 days, scratching and skin parameters (transepidermal water loss (TEWL), hydration, and local blood flow) were evaluated before and after dorsal skin exposure to acetone to induce the itch-related dry skin. At the end of the study, animals were sacrificed, and skin samples collected for fatty acids composition analysis by GC-FID.Results: FO supplementation reduced the TEWL and increased the skin hydration, with significant changes from day 60 on, while skin microcirculation registered no changes. It also alleviated the acetone induced skin barrier alteration, revealed by a faster resolution of TEWL and hydration, and elimination of itch-related scratching induced by dry skin. These changes were associated with the shift in the skin fatty acids incorporation pattern (richer in n-3 with n-6/n-3 < 5) resulting from the FO supplementation.Conclusion: Skin barrier dynamics seem to be influenced by FO n-3 PUFA, with suppressive effects on the scratching behaviour induced by dry skin. Hence, long-term supplementation with n-3 PUFA rich nutrients might reinforce and restore cutaneous integrity and function. (C) 2015 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.