The human gonadotropin-releasing hormone receptor gene (GNRHR) maps to chromosome band 4q13.
The human gonadotropin-releasing hormone receptor gene (GNRHR) maps to chromosome band 4q13.
复制标题
人类促性腺激素释放激素受体基因 (GNRHR) 定位于染色体带 4q13。
DOI：
10.1159/000134035
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Neill,JD
中科院分区：
文献类型：
--
作者：
Kakar,SS;Neill,JD
代表高亲和力促性腺激素释放激素(GnRH)受体的基因已从人脑下垂体、乳腺肿瘤细胞系(MCF-7)和卵巢肿瘤中分子克隆出来。测定了该基因的核苷酸序列，并证明了其在多种人类肿瘤和肿瘤细胞系中的表达。在这项研究中，我们通过对人与仓鼠体细胞杂交的基因组DNA的聚合酶链式反应(PCR)分析，将GnRH受体基因定位在人的4号染色体上。通过与GnRH受体基因(GNRHR)的荧光原位杂交，将该基因亚区块化到染色体4Q13带。
A cDNA representing the high-affinity gonadotropin-releasing hormone (GnRH) receptor has been molecularly cloned from the human pituitary gland, a breast tumor cell line (MCF 7), and an ovarian tumor. The nucleotide sequence of this cDNA was determined, and its expression in various human tumors and tumor cell lines was demonstrated. In this study, we localized the gene encoding the GnRH receptor to human chromosome 4, using polymerase chain reaction (PCR) analysis of genomic DNA from human × hamster somatic cell hybrids. The gene was sublocalized to chromosome band 4q13 using fluorescence in situ hybridization with the GnRH receptor gene (GNRHR).