Characteristics common to a cytokine family spanning five orders of insects
Characteristics common to a cytokine family spanning five orders of insects
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DOI：
10.1016/j.ibmb.2012.03.001
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发表时间：
2012-06-01
影响因子：
3.8
通讯作者：
Hayakawa, Yoichi
中科院分区：
文献类型：
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作者：
Matsumoto, Hitoshi;Tsuzuki, Seiji;Hayakawa, Yoichi
生长阻断肽（Growth-blocking peptide，GBP）是昆虫细胞因子家族的一员，具有多种功能，包括生长和免疫控制。该细胞因子家族的成员已在15种鳞翅目昆虫中被报道，我们最近在双翅目昆虫如绿蝇和黑腹果蝇中鉴定出GBP-like肽，表明该肽家族不是鳞翅目昆虫特有的。为了扩展我们对该肽家族的了解，我们从拟步甲昆虫Zophobas atratus中纯化了相同家族的肽，（1）分离了其cDNA，并对其进行了测序。Zophobas atratus的GBP序列与报道的来自相同家族的肽的序列数据一起使我们能够对几种昆虫的EST和基因组数据库进行BLAST搜索，这些昆虫包括鞘翅目、双翅目、膜翅目、和半翅目昆虫的同源肽基因。在这里，我们报告保守的结构特征，在这些序列数据。它们由编码在73-152个氨基酸的前体的C末端的19-30个氨基酸残基组成，并且含有基序C-x（2）-G-x（4，6）-G-x（1，2）-C-[KR]，其与哺乳动物EGF肽家族中的基序具有一定的相似性。这些数据表明，这些属于一个家庭的小细胞因子存在于至少五个昆虫目。(C)2012爱思唯尔有限公司保留所有权利。
Growth-blocking peptide (GBP) is a member of an insect cytokine family with diverse functions including growth and immunity controls. Members of this cytokine family have been reported in 15 species of Lepidoptera, and we have recently identified GBP-like peptides in Diptera such as Lucilia cuprina and Drosophila melanogaster, indicating that this peptide family is not specific to Lepidoptera. In order to extend our knowledge of this peptide family, we purified the same family peptide from one of the tenebrionids, Zophobas atratus,(1) isolated its cDNA, and sequenced it. The Z atratus GBP sequence together with reported sequence data of peptides from the same family enabled us to perform BLAST searches against EST and genome databases of several insect species including Coleoptera, Diptera, Hymenoptera, and Hemiptera and identify homologous peptide genes. Here we report conserved structural features in these sequence data. They consist of 19-30 amino acid residues encoded at the C terminus of a 73-152 amino acid precursor and contain the motif C-x(2)-G-x(4,6)-G-x(1,2)-C-[KR], which shares a certain similarity with the motif in the mammalian EGF peptide family. These data indicate that these small cytokines belonging to one family are present in at least five insect orders. (C) 2012 Elsevier Ltd. All rights reserved.