Single-Nucleotide Polymorphisms of IL-17 Gene Are Associated with Asthma Susceptibility in an Asian Population.
Single-Nucleotide Polymorphisms of IL-17 Gene Are Associated with Asthma Susceptibility in an Asian Population.
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DOI：
10.12659/msm.895494
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发表时间：
2016-03-08
期刊：
影响因子：
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通讯作者：
Cai S
中科院分区：
文献类型：
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作者：
Du J;Han JC;Zhang YJ;Qi GB;Li HB;Zhang YJ;Cai S
本研究的目的是检测白细胞介素-17（IL-17）的单核苷酸多态性（SNPs），包括rs763780（7488 A/G）、rs 2275913（-197G/A）和rs 8193036（-737C/T）与亚洲人群哮喘易感性之间的关系。选择2013年10月至2014年12月在我院住院的125例哮喘患者作为病例组。另选取在我院体检的健康体检者132例作为对照组。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测IL-17 rs763780、rs 2275913和rs 8193036 SNP的基因型频率。采用综合Meta分析2.0（CMA 2.0）软件进行Meta分析。结果表明，哮喘患者IL-17基因rs 2275913位点GA基因型和rs 8193036位点TT基因型频率高于健康对照组（均P<0.001）。IL-17 rs763780基因型频率在哮喘组和健康对照组间差异无统计学意义（P>0.05）。哮喘组与健康对照组rs 2275913和rs 8193036基因频率比较，在等位基因和成瘾模型中均具有统计学意义（均P<0.05）。哮喘患者和健康对照组IL-17 rs763780基因频率在等位基因模型中差异有统计学意义（P<0.05），而在成瘾模型中差异无统计学意义（P>0.05）。我们的病例对照研究的总体结果得到了荟萃分析的进一步证实。我们的结果表明，在亚洲人群中，IL-17 rs763780、rs 2275913和rs 8193036 SNP可能与哮喘易感性相关，IL-17 rs 2275913中的GA基因型和rs 8193036中的TT基因型可能导致亚洲人哮喘风险增加。
The aim of this study was to examine the associations between the single-nucleotide polymorphisms (SNPs) of interleukin-17 (IL-17), including rs763780 (7488A/G), rs2275913 (–197G/A), and rs8193036 (–737C/T), and asthma susceptibility in an Asian population. From Oct 2013 to Dec 2014, 125 asthma patients enrolled in our hospital were selected as the case group. Another 132 healthy controls undergoing physical examinations in our hospital were enrolled as the control group. The genotype frequencies of IL-17 rs763780, rs2275913 and rs8193036 SNPs were detected using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Comprehensive Meta-analysis 2.0 (CMA 2.0) software was applied for meta-analysis. Our results demonstrated that asthma patients presented with higher frequencies of GA genotype in rs2275913 and TT genotype in rs8193036 of IL-17 than healthy controls (both P<0.001). The genotype frequencies of IL-17 rs763780 between the asthma patients and healthy controls exhibited no significant differences (P>0.05). The comparisons on the rs2275913 and rs8193036 frequencies between the asthma patients and healthy controls were statistically significant in both allele and addictive models (all P<0.05). The frequency of IL-17 rs763780 between the asthma patients and healthy controls were statistically different in allele models (P<0.05), but not in addictive models (P>0.05). The overall results of our case-control study were further confirmed by meta-analysis. Our results revealed that, in an Asian population, IL-17 rs763780, rs2275913, and rs8193036 SNPs may be associated with asthma susceptibility, and GA genotype in rs2275913 and TT genotype in rs8193036 of IL-17 may contribute to increased risk of asthma in Asians.